High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging
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- High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging
Final published version, 1.01 MB, PDF document
The crystal violet assay is widely used for biofilm quantitation despite its toxicity and variability. Here, we instead combine fluorescence labelling with the Cytation 5 multi-mode plate reader, to enable simultaneous acquisition of both quantitative and imaging biofilm data. This high-throughput method produces more robust data and provides information about morphology and spatial species organization within the biofilm.
Original language | English |
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Article number | 106343 |
Journal | Journal of Microbiological Methods |
Volume | 190 |
Number of pages | 5 |
ISSN | 0167-7012 |
DOIs | |
Publication status | Published - 2021 |
Bibliographical note
Copyright © 2021. Published by Elsevier B.V.
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