Identification of the gene (aroK) encoding shikimic acid kinase I of Escherichia coli
Research output: Contribution to journal › Journal article › Research › peer-review
DNA sequence analysis has revealed that an unidentified open reading frame (ufr1) is present immediately preceding the aroB gene of Escherichia coli. The predicted protein product of urf1 contains a consensus ATP-binding-site sequence and shows 34% amino acid homology to shikimate kinase II in a 97-amino-acid region. Inactivation of urf1 by insertion of an antibiotic resistance gene had a polar effect on aroB, indicating that these two genes constitute a transcriptional unit. The auxotrophic requirements of a strain mutant for both urf1 and aroL (encoding shikimate kinase II) are consistent with shikimate kinase deficiency. We propose that urf1 encodes shikimate kinase I and that it be designated aroK.
Original language | English |
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Journal | Journal of Bacteriology |
Volume | 174 |
Issue number | 2 |
Pages (from-to) | 525-9 |
Number of pages | 5 |
ISSN | 0021-9193 |
Publication status | Published - Jan 1992 |
- Amino Acid Sequence, Base Sequence, Chromosomes, Bacterial, Cloning, Molecular, DNA Transposable Elements, Escherichia coli/enzymology, Genes, Bacterial, Lac Operon, Molecular Sequence Data, Mutation, Open Reading Frames, Phosphotransferases/genetics, Phosphotransferases (Alcohol Group Acceptor), Transcription, Genetic
Research areas
ID: 200973058