TY - JOUR

T1 - In vivo, genome-wide profiling of endogenously tagged chromatin-binding proteins with spatial and temporal resolution using NanoDam in Drosophila

AU - Tang, Jocelyn L.Y.

AU - Krautz, Robert

AU - Llorà-Batlle, Oriol

AU - Hakes, Anna E.

AU - Fox, Paul M.

AU - Brand, Andrea H.

N1 - Publisher Copyright: © 2022 The Authors

PY - 2022

Y1 - 2022

N2 - NanoDam is a technique for genome-wide profiling of the binding targets of any endogenously tagged chromatin-binding protein in vivo, without the need for antibodies, crosslinking, or immunoprecipitation. Here, we explain the procedure for NanoDam experiments in Drosophila, starting from a genetic cross, to the generation of sequencing libraries and, finally, bioinformatic analysis. This protocol can be readily adapted for use in other model systems after simple modifications. For complete details on the use and execution of this protocol, please refer to Tang et al. (2022).

AB - NanoDam is a technique for genome-wide profiling of the binding targets of any endogenously tagged chromatin-binding protein in vivo, without the need for antibodies, crosslinking, or immunoprecipitation. Here, we explain the procedure for NanoDam experiments in Drosophila, starting from a genetic cross, to the generation of sequencing libraries and, finally, bioinformatic analysis. This protocol can be readily adapted for use in other model systems after simple modifications. For complete details on the use and execution of this protocol, please refer to Tang et al. (2022).

KW - Cell biology

KW - Developmental biology

KW - Genomics

KW - Model organisms

KW - Sequencing

U2 - 10.1016/j.xpro.2022.101788

DO - 10.1016/j.xpro.2022.101788

M3 - Journal article

C2 - 36345375

AN - SCOPUS:85141893877

VL - 3

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 4

M1 - 101788

ER -