Interleukin 1-induced down-regulation of antibody binding to CD4 molecules on human lymphocytes
Research output: Contribution to journal › Journal article › Research › peer-review
Interleukin 1 (IL-1) is involved in the early activation of T lymphocytes. The CD4 antigen, described as a phenotypic marker of helper T cells, is also important in early T-cell activation by its ability to bind to MHC class II molecules on antigen-presenting cells, and to transmit positive (and negative) signals to the cells. We observed that purified human monocyte IL-1 as well as recombinant IL-1 alpha and IL-1 beta selectively decreased the binding of monoclonal antibodies to CD4 on the surface of otherwise unstimulated blood T cells, in contrast to prestimulated and continuously grown CD4+ cells. Under optimal growth conditions, the initial reduction in antibody binding to CD4 was followed by an apparent re-expression of the CD4 antigen even in the presence of high concentrations of IL-1. This re-expression did not occur if the cells were cultured at 4 degrees C, or after treatment with actinomycin D or cytochalasin B, indicating that protein synthesis and intact microfilament function were essential for re-expression of CD4 binding. The mechanism by which CD4 molecules are physically and/or functionally modulated by IL-1 is unclear.
Original language | English |
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Journal | Scandinavian Journal of Immunology |
Volume | 27 |
Issue number | 6 |
Pages (from-to) | 679-84 |
Number of pages | 5 |
ISSN | 0300-9475 |
Publication status | Published - 1988 |
Bibliographical note
Keywords: Antibodies, Monoclonal; Antigen-Antibody Reactions; Antigens, Differentiation, T-Lymphocyte; Flow Cytometry; Humans; Interleukin-1; Isoantibodies; Lymphocyte Activation; T-Lymphocytes
ID: 10637698