Interleukin 1-induced down-regulation of antibody binding to CD4 molecules on human lymphocytes
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Interleukin 1-induced down-regulation of antibody binding to CD4 molecules on human lymphocytes. / Tvede, N; Christensen, L D; Ødum, Niels; Wiik, A; Bendtzen, K.
In: Scandinavian Journal of Immunology, Vol. 27, No. 6, 1988, p. 679-84.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Interleukin 1-induced down-regulation of antibody binding to CD4 molecules on human lymphocytes
AU - Tvede, N
AU - Christensen, L D
AU - Ødum, Niels
AU - Wiik, A
AU - Bendtzen, K
N1 - Keywords: Antibodies, Monoclonal; Antigen-Antibody Reactions; Antigens, Differentiation, T-Lymphocyte; Flow Cytometry; Humans; Interleukin-1; Isoantibodies; Lymphocyte Activation; T-Lymphocytes
PY - 1988
Y1 - 1988
N2 - Interleukin 1 (IL-1) is involved in the early activation of T lymphocytes. The CD4 antigen, described as a phenotypic marker of helper T cells, is also important in early T-cell activation by its ability to bind to MHC class II molecules on antigen-presenting cells, and to transmit positive (and negative) signals to the cells. We observed that purified human monocyte IL-1 as well as recombinant IL-1 alpha and IL-1 beta selectively decreased the binding of monoclonal antibodies to CD4 on the surface of otherwise unstimulated blood T cells, in contrast to prestimulated and continuously grown CD4+ cells. Under optimal growth conditions, the initial reduction in antibody binding to CD4 was followed by an apparent re-expression of the CD4 antigen even in the presence of high concentrations of IL-1. This re-expression did not occur if the cells were cultured at 4 degrees C, or after treatment with actinomycin D or cytochalasin B, indicating that protein synthesis and intact microfilament function were essential for re-expression of CD4 binding. The mechanism by which CD4 molecules are physically and/or functionally modulated by IL-1 is unclear.
AB - Interleukin 1 (IL-1) is involved in the early activation of T lymphocytes. The CD4 antigen, described as a phenotypic marker of helper T cells, is also important in early T-cell activation by its ability to bind to MHC class II molecules on antigen-presenting cells, and to transmit positive (and negative) signals to the cells. We observed that purified human monocyte IL-1 as well as recombinant IL-1 alpha and IL-1 beta selectively decreased the binding of monoclonal antibodies to CD4 on the surface of otherwise unstimulated blood T cells, in contrast to prestimulated and continuously grown CD4+ cells. Under optimal growth conditions, the initial reduction in antibody binding to CD4 was followed by an apparent re-expression of the CD4 antigen even in the presence of high concentrations of IL-1. This re-expression did not occur if the cells were cultured at 4 degrees C, or after treatment with actinomycin D or cytochalasin B, indicating that protein synthesis and intact microfilament function were essential for re-expression of CD4 binding. The mechanism by which CD4 molecules are physically and/or functionally modulated by IL-1 is unclear.
M3 - Journal article
C2 - 3260682
VL - 27
SP - 679
EP - 684
JO - Scandinavian Journal of Immunology, Supplement
JF - Scandinavian Journal of Immunology, Supplement
SN - 0301-6323
IS - 6
ER -
ID: 10637698