Mapping the double-strand breaks at the mating-type locus in fission yeast by genomic sequencing

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Mapping the double-strand breaks at the mating-type locus in fission yeast by genomic sequencing. / Nielsen, O; Egel, R; Nielsen, Olaf.

In: E M B O Journal, Vol. 8, No. 1, 01.01.1989, p. 269-76.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Nielsen, O, Egel, R & Nielsen, O 1989, 'Mapping the double-strand breaks at the mating-type locus in fission yeast by genomic sequencing', E M B O Journal, vol. 8, no. 1, pp. 269-76.

APA

Nielsen, O., Egel, R., & Nielsen, O. (1989). Mapping the double-strand breaks at the mating-type locus in fission yeast by genomic sequencing. E M B O Journal, 8(1), 269-76.

Vancouver

Nielsen O, Egel R, Nielsen O. Mapping the double-strand breaks at the mating-type locus in fission yeast by genomic sequencing. E M B O Journal. 1989 Jan 1;8(1):269-76.

Author

Nielsen, O ; Egel, R ; Nielsen, Olaf. / Mapping the double-strand breaks at the mating-type locus in fission yeast by genomic sequencing. In: E M B O Journal. 1989 ; Vol. 8, No. 1. pp. 269-76.

Bibtex

@article{58bf9185c3154bb2b063470beb5ecad0,
title = "Mapping the double-strand breaks at the mating-type locus in fission yeast by genomic sequencing",
abstract = "In fission yeast mating-type switching is initiated by the formation of a double-strand DNA break at the mating-type locus. A prerequisite for generation of the break is some 'imprinting' of the DNA in the previous cell cycle. We have used the technique of genomic sequencing to map the position of the break directly on chromosomal DNA cleaved in vivo. On one strand the break is situated very close to the right-hand border of the expressed mat1 cassette. Cells of opposite mating type, P and M, have their breaks at slightly different positions on this strand. On the other DNA strand of both alleles the ends are probably masked by tightly bound proteins and therefore the precise nature of the break could not be determined. Since the break is stable throughout the cell cycle, these proteins may function in vivo to confer structural stability on the chromosomes having the break. The implications of these findings for models of mating-type switching are discussed.",
keywords = "Base Sequence, Chromosome Mapping, DNA, DNA, Fungal, Genes, Fungal, Genes, Mating Type, Fungal, Molecular Sequence Data, Peptides, Recombination, Genetic, Restriction Mapping, Saccharomycetales, Schizosaccharomyces, Sequence Homology, Nucleic Acid",
author = "O Nielsen and R Egel and Olaf Nielsen",
year = "1989",
month = jan,
day = "1",
language = "English",
volume = "8",
pages = "269--76",
journal = "E M B O Journal",
issn = "0261-4189",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Mapping the double-strand breaks at the mating-type locus in fission yeast by genomic sequencing

AU - Nielsen, O

AU - Egel, R

AU - Nielsen, Olaf

PY - 1989/1/1

Y1 - 1989/1/1

N2 - In fission yeast mating-type switching is initiated by the formation of a double-strand DNA break at the mating-type locus. A prerequisite for generation of the break is some 'imprinting' of the DNA in the previous cell cycle. We have used the technique of genomic sequencing to map the position of the break directly on chromosomal DNA cleaved in vivo. On one strand the break is situated very close to the right-hand border of the expressed mat1 cassette. Cells of opposite mating type, P and M, have their breaks at slightly different positions on this strand. On the other DNA strand of both alleles the ends are probably masked by tightly bound proteins and therefore the precise nature of the break could not be determined. Since the break is stable throughout the cell cycle, these proteins may function in vivo to confer structural stability on the chromosomes having the break. The implications of these findings for models of mating-type switching are discussed.

AB - In fission yeast mating-type switching is initiated by the formation of a double-strand DNA break at the mating-type locus. A prerequisite for generation of the break is some 'imprinting' of the DNA in the previous cell cycle. We have used the technique of genomic sequencing to map the position of the break directly on chromosomal DNA cleaved in vivo. On one strand the break is situated very close to the right-hand border of the expressed mat1 cassette. Cells of opposite mating type, P and M, have their breaks at slightly different positions on this strand. On the other DNA strand of both alleles the ends are probably masked by tightly bound proteins and therefore the precise nature of the break could not be determined. Since the break is stable throughout the cell cycle, these proteins may function in vivo to confer structural stability on the chromosomes having the break. The implications of these findings for models of mating-type switching are discussed.

KW - Base Sequence

KW - Chromosome Mapping

KW - DNA

KW - DNA, Fungal

KW - Genes, Fungal

KW - Genes, Mating Type, Fungal

KW - Molecular Sequence Data

KW - Peptides

KW - Recombination, Genetic

KW - Restriction Mapping

KW - Saccharomycetales

KW - Schizosaccharomyces

KW - Sequence Homology, Nucleic Acid

M3 - Journal article

C2 - 2714252

VL - 8

SP - 269

EP - 276

JO - E M B O Journal

JF - E M B O Journal

SN - 0261-4189

IS - 1

ER -

ID: 33577652