Mik1 levels accumulate in S phase and may mediate an intrinsic link between S phase and mitosis

Research output: Contribution to journal › Journal article › Research › peer-review

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Mik1 levels accumulate in S phase and may mediate an intrinsic link between S phase and mitosis. / Christensen, P U; Bentley, N J; Martinho, R G; Nielsen, O; Carr, Antony; Nielsen, Olaf.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 6, 14.03.2000, p. 2579-84.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Christensen, PU, Bentley, NJ, Martinho, RG, Nielsen, O, Carr, A & Nielsen, O 2000, 'Mik1 levels accumulate in S phase and may mediate an intrinsic link between S phase and mitosis', Proceedings of the National Academy of Sciences of the United States of America, vol. 97, no. 6, pp. 2579-84.

APA

Christensen, P. U., Bentley, N. J., Martinho, R. G., Nielsen, O., Carr, A., & Nielsen, O. (2000). Mik1 levels accumulate in S phase and may mediate an intrinsic link between S phase and mitosis. Proceedings of the National Academy of Sciences of the United States of America, 97(6), 2579-84.

Vancouver

Christensen PU, Bentley NJ, Martinho RG, Nielsen O, Carr A, Nielsen O. Mik1 levels accumulate in S phase and may mediate an intrinsic link between S phase and mitosis. Proceedings of the National Academy of Sciences of the United States of America. 2000 Mar 14;97(6):2579-84.

Author

Christensen, P U ; Bentley, N J ; Martinho, R G ; Nielsen, O ; Carr, Antony ; Nielsen, Olaf. / Mik1 levels accumulate in S phase and may mediate an intrinsic link between S phase and mitosis. In: Proceedings of the National Academy of Sciences of the United States of America. 2000 ; Vol. 97, No. 6. pp. 2579-84.

Bibtex

@article{5000a57ac7544ca4bc03b1f7a23ba245,
title = "Mik1 levels accumulate in S phase and may mediate an intrinsic link between S phase and mitosis",
abstract = "Two paradigms exist for maintaining order during cell-cycle progression: intrinsic controls, where passage through one part of the cell cycle directly affects the ability to execute another, and checkpoint controls, where external pathways impose order in response to aberrant structures. By studying the mitotic inhibitor Mik1, we have identified evidence for an intrinsic link between unperturbed S phase and mitosis. We propose a model in which S/M linkage can be generated by the production and stabilization of Mik1 protein during S phase. The production of Mik1 during unperturbed S phase is independent of the Rad3- and Cds1-dependent checkpoint controls. In response to perturbed S phase, Rad3-Cds1 checkpoint controls are required to maintain high levels of Mik1, probably indirectly by extending the S phase period, where Mik1 is stable. In addition, we find that Mik1 protein can be moderately induced in response to irradiation of G(2) cells in a Chk1-dependent manner.",
keywords = "Enzyme Inhibitors, Epitopes, Flow Cytometry, G2 Phase, Hydroxyurea, Immunoblotting, Microscopy, Fluorescence, Mitosis, Models, Biological, Phosphoric Monoester Hydrolases, Phosphorylation, Protein Kinases, Protein-Tyrosine Kinases, S Phase, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Time Factors",
author = "Christensen, {P U} and Bentley, {N J} and Martinho, {R G} and O Nielsen and Antony Carr and Olaf Nielsen",
year = "2000",
month = mar,
day = "14",
language = "English",
volume = "97",
pages = "2579--84",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "The National Academy of Sciences of the United States of America",
number = "6",

}

RIS

TY - JOUR

T1 - Mik1 levels accumulate in S phase and may mediate an intrinsic link between S phase and mitosis

AU - Christensen, P U

AU - Bentley, N J

AU - Martinho, R G

AU - Nielsen, O

AU - Carr, Antony

AU - Nielsen, Olaf

PY - 2000/3/14

Y1 - 2000/3/14

N2 - Two paradigms exist for maintaining order during cell-cycle progression: intrinsic controls, where passage through one part of the cell cycle directly affects the ability to execute another, and checkpoint controls, where external pathways impose order in response to aberrant structures. By studying the mitotic inhibitor Mik1, we have identified evidence for an intrinsic link between unperturbed S phase and mitosis. We propose a model in which S/M linkage can be generated by the production and stabilization of Mik1 protein during S phase. The production of Mik1 during unperturbed S phase is independent of the Rad3- and Cds1-dependent checkpoint controls. In response to perturbed S phase, Rad3-Cds1 checkpoint controls are required to maintain high levels of Mik1, probably indirectly by extending the S phase period, where Mik1 is stable. In addition, we find that Mik1 protein can be moderately induced in response to irradiation of G(2) cells in a Chk1-dependent manner.

AB - Two paradigms exist for maintaining order during cell-cycle progression: intrinsic controls, where passage through one part of the cell cycle directly affects the ability to execute another, and checkpoint controls, where external pathways impose order in response to aberrant structures. By studying the mitotic inhibitor Mik1, we have identified evidence for an intrinsic link between unperturbed S phase and mitosis. We propose a model in which S/M linkage can be generated by the production and stabilization of Mik1 protein during S phase. The production of Mik1 during unperturbed S phase is independent of the Rad3- and Cds1-dependent checkpoint controls. In response to perturbed S phase, Rad3-Cds1 checkpoint controls are required to maintain high levels of Mik1, probably indirectly by extending the S phase period, where Mik1 is stable. In addition, we find that Mik1 protein can be moderately induced in response to irradiation of G(2) cells in a Chk1-dependent manner.

KW - Enzyme Inhibitors

KW - Epitopes

KW - Flow Cytometry

KW - G2 Phase

KW - Hydroxyurea

KW - Immunoblotting

KW - Microscopy, Fluorescence

KW - Mitosis

KW - Models, Biological

KW - Phosphoric Monoester Hydrolases

KW - Phosphorylation

KW - Protein Kinases

KW - Protein-Tyrosine Kinases

KW - S Phase

KW - Schizosaccharomyces

KW - Schizosaccharomyces pombe Proteins

KW - Time Factors

M3 - Journal article

C2 - 10716991

VL - 97

SP - 2579

EP - 2584

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 6

ER -

ID: 33576861