Molecular anatomy of a skin gland: histochemical and biochemical investigations on the mucous glands of Xenopus laevis
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Molecular anatomy of a skin gland : histochemical and biochemical investigations on the mucous glands of Xenopus laevis. / Schumacher, U; Adam, E; Hauser, F; Probst, J C; Hoffmann, W.
In: Journal of Histochemistry and Cytochemistry, Vol. 42, No. 1, 01.1994, p. 57-65.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Molecular anatomy of a skin gland
T2 - histochemical and biochemical investigations on the mucous glands of Xenopus laevis
AU - Schumacher, U
AU - Adam, E
AU - Hauser, F
AU - Probst, J C
AU - Hoffmann, W
PY - 1994/1
Y1 - 1994/1
N2 - The purpose of this study was to investigate the structure and chemical composition of the mucous skin gland of Xenopus laevis by combined morphological and biochemical techniques. Protein backbones of mucins were localized immunohistochemically in the gland with anti-peptide antibodies. Acid mucins were demonstrated by conventional histochemical techniques and their terminal carbohydrate residues were localized by lectin histochemistry. A close correlation between antibody and lectin binding of the same glycoproteins was achieved on Western blots from isolated skin gland mucins, indicating that the lectin-binding sites were due to defined mucin molecules. The cone cell, thought to be a degenerative cell in the past, contained mucin granules with an electron-dense core, strong PAS reactivity, a special lectin-binding pattern, and localization of integumentary mucins FIM-B.1 and FIM-C.1. These results indicate that cone cells are a distinct cell type, elaborating and releasing particular mucins, and that functional heterogeneity of mucus-producing cells exists in the mucous skin glands of X. laevis.
AB - The purpose of this study was to investigate the structure and chemical composition of the mucous skin gland of Xenopus laevis by combined morphological and biochemical techniques. Protein backbones of mucins were localized immunohistochemically in the gland with anti-peptide antibodies. Acid mucins were demonstrated by conventional histochemical techniques and their terminal carbohydrate residues were localized by lectin histochemistry. A close correlation between antibody and lectin binding of the same glycoproteins was achieved on Western blots from isolated skin gland mucins, indicating that the lectin-binding sites were due to defined mucin molecules. The cone cell, thought to be a degenerative cell in the past, contained mucin granules with an electron-dense core, strong PAS reactivity, a special lectin-binding pattern, and localization of integumentary mucins FIM-B.1 and FIM-C.1. These results indicate that cone cells are a distinct cell type, elaborating and releasing particular mucins, and that functional heterogeneity of mucus-producing cells exists in the mucous skin glands of X. laevis.
KW - Animals
KW - Blotting, Western
KW - Electrophoresis, Polyacrylamide Gel
KW - Exocrine Glands/anatomy & histology
KW - Female
KW - Immunohistochemistry
KW - Lectins
KW - Microscopy, Electron
KW - Mucus/chemistry
KW - Nuclear Proteins/analysis
KW - Proliferating Cell Nuclear Antigen
KW - Skin/anatomy & histology
KW - Xenopus laevis/anatomy & histology
U2 - 10.1177/42.1.7903329
DO - 10.1177/42.1.7903329
M3 - Journal article
C2 - 7903329
VL - 42
SP - 57
EP - 65
JO - Journal of Histochemistry and Cytochemistry
JF - Journal of Histochemistry and Cytochemistry
SN - 0022-1554
IS - 1
ER -
ID: 347885802