Molecular size and charge characterization by rheophoresis I. Theory and gel calibration

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Molecular size and charge characterization by rheophoresis I. Theory and gel calibration. / Waldmann‐Meyer, Henrik; Pedersen, Per Amstrup.

In: Electrophoresis, Vol. 5, No. 1, 01.01.1984, p. 18-21.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Waldmann‐Meyer, H & Pedersen, PA 1984, 'Molecular size and charge characterization by rheophoresis I. Theory and gel calibration', Electrophoresis, vol. 5, no. 1, pp. 18-21. https://doi.org/10.1002/elps.1150050103

APA

Waldmann‐Meyer, H., & Pedersen, P. A. (1984). Molecular size and charge characterization by rheophoresis I. Theory and gel calibration. Electrophoresis, 5(1), 18-21. https://doi.org/10.1002/elps.1150050103

Vancouver

Waldmann‐Meyer H, Pedersen PA. Molecular size and charge characterization by rheophoresis I. Theory and gel calibration. Electrophoresis. 1984 Jan 1;5(1):18-21. https://doi.org/10.1002/elps.1150050103

Author

Waldmann‐Meyer, Henrik ; Pedersen, Per Amstrup. / Molecular size and charge characterization by rheophoresis I. Theory and gel calibration. In: Electrophoresis. 1984 ; Vol. 5, No. 1. pp. 18-21.

Bibtex

@article{3efbaf1c5dc2486e8260da47ad6273ca,
title = "Molecular size and charge characterization by rheophoresis I. Theory and gel calibration",
abstract = "Rheophoresis, i.e. thin‐layer electrophoresis in particulate gels with controlled evaporation, combines in one experiment the chromatographic and the electrophoretic characteristics of macromlecules in such a way that they can be determined independently of each other. In this paper theory and experimental corroboration for molecular size determination are presented. We describe the migration velocity of any molecule as the sum of an electrophoretic, an electroosmotic and a rheophoretic component. The latter is the velocity originating from the liquid flow produced by evaporation. It is a function of the position along the gel axis and the cross‐section available to the molecule, but totally independent of the molecular charge. As a result of the position dependence, the rheophoretic component can be isolated from the two others and the available cross‐section determined by means of a simple plot. Thus, the chromatographic partition coefficient is readily calculated and the effective hydrodynamic radius of the molecule obtained.",
author = "Henrik Waldmann‐Meyer and Pedersen, {Per Amstrup}",
year = "1984",
month = jan,
day = "1",
doi = "10.1002/elps.1150050103",
language = "English",
volume = "5",
pages = "18--21",
journal = "Electrophoresis",
issn = "0173-0835",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "1",

}

RIS

TY - JOUR

T1 - Molecular size and charge characterization by rheophoresis I. Theory and gel calibration

AU - Waldmann‐Meyer, Henrik

AU - Pedersen, Per Amstrup

PY - 1984/1/1

Y1 - 1984/1/1

N2 - Rheophoresis, i.e. thin‐layer electrophoresis in particulate gels with controlled evaporation, combines in one experiment the chromatographic and the electrophoretic characteristics of macromlecules in such a way that they can be determined independently of each other. In this paper theory and experimental corroboration for molecular size determination are presented. We describe the migration velocity of any molecule as the sum of an electrophoretic, an electroosmotic and a rheophoretic component. The latter is the velocity originating from the liquid flow produced by evaporation. It is a function of the position along the gel axis and the cross‐section available to the molecule, but totally independent of the molecular charge. As a result of the position dependence, the rheophoretic component can be isolated from the two others and the available cross‐section determined by means of a simple plot. Thus, the chromatographic partition coefficient is readily calculated and the effective hydrodynamic radius of the molecule obtained.

AB - Rheophoresis, i.e. thin‐layer electrophoresis in particulate gels with controlled evaporation, combines in one experiment the chromatographic and the electrophoretic characteristics of macromlecules in such a way that they can be determined independently of each other. In this paper theory and experimental corroboration for molecular size determination are presented. We describe the migration velocity of any molecule as the sum of an electrophoretic, an electroosmotic and a rheophoretic component. The latter is the velocity originating from the liquid flow produced by evaporation. It is a function of the position along the gel axis and the cross‐section available to the molecule, but totally independent of the molecular charge. As a result of the position dependence, the rheophoretic component can be isolated from the two others and the available cross‐section determined by means of a simple plot. Thus, the chromatographic partition coefficient is readily calculated and the effective hydrodynamic radius of the molecule obtained.

UR - http://www.scopus.com/inward/record.url?scp=84988129079&partnerID=8YFLogxK

U2 - 10.1002/elps.1150050103

DO - 10.1002/elps.1150050103

M3 - Journal article

AN - SCOPUS:84988129079

VL - 5

SP - 18

EP - 21

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 1

ER -

ID: 227044377