Phage-Inducible Chromosomal Islands as a Diagnostic Platform to Capture and Detect Bacterial Pathogens

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Phage-Inducible Chromosomal Islands as a Diagnostic Platform to Capture and Detect Bacterial Pathogens. / Ibarra-Chávez, Rodrigo; Reboud, Julien; Penadés, José R; Cooper, Jonathan M.

In: Advanced Science, Vol. 10, No. 24, 2301643, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Ibarra-Chávez, R, Reboud, J, Penadés, JR & Cooper, JM 2023, 'Phage-Inducible Chromosomal Islands as a Diagnostic Platform to Capture and Detect Bacterial Pathogens', Advanced Science, vol. 10, no. 24, 2301643. https://doi.org/10.1002/advs.202301643

APA

Ibarra-Chávez, R., Reboud, J., Penadés, J. R., & Cooper, J. M. (2023). Phage-Inducible Chromosomal Islands as a Diagnostic Platform to Capture and Detect Bacterial Pathogens. Advanced Science, 10(24), [2301643]. https://doi.org/10.1002/advs.202301643

Vancouver

Ibarra-Chávez R, Reboud J, Penadés JR, Cooper JM. Phage-Inducible Chromosomal Islands as a Diagnostic Platform to Capture and Detect Bacterial Pathogens. Advanced Science. 2023;10(24). 2301643. https://doi.org/10.1002/advs.202301643

Author

Ibarra-Chávez, Rodrigo ; Reboud, Julien ; Penadés, José R ; Cooper, Jonathan M. / Phage-Inducible Chromosomal Islands as a Diagnostic Platform to Capture and Detect Bacterial Pathogens. In: Advanced Science. 2023 ; Vol. 10, No. 24.

Bibtex

@article{506b4a5e96844093a7212ec3ad70dfa7,

title = "Phage-Inducible Chromosomal Islands as a Diagnostic Platform to Capture and Detect Bacterial Pathogens",

abstract = "Phage-inducible chromosomal islands (PICIs) are a family of phage satellites that hijack phage components to facilitate their mobility and spread. Recently, these genetic constructs are repurposed as antibacterial drones, enabling a new toolbox for unorthodox applications in biotechnology. To illustrate a new suite of functions, the authors have developed a user-friendly diagnostic system, based upon PICI transduction to selectively enrich bacteria, allowing the detection and sequential recovery of Escherichia coli and Staphylococcus aureus. The system enables high transfer rates and sensitivities in comparison with phages, with detection down to ≈50 CFU mL -1 . In contrast to conventional detection strategies, which often rely on nucleic acid molecular assays, and cannot differentiate between dead and live organisms, this approach enables visual sensing of viable pathogens only, through the expression of a reporter gene encoded in the PICI. The approach extends diagnostic sensing mechanisms beyond cell-free synthetic biology strategies, enabling new synthetic biology/biosensing toolkits. ",

author = "Rodrigo Ibarra-Ch{\'a}vez and Julien Reboud and Penad{\'e}s, {Jos{\'e} R} and Cooper, {Jonathan M}",

note = "{\textcopyright} 2023 The Authors. Advanced Science published by Wiley-VCH GmbH.",

year = "2023",

doi = "10.1002/advs.202301643",

language = "English",

volume = "10",

journal = "Advanced Science",

issn = "2198-3844",

publisher = "Wiley-VCH",

number = "24",

}

RIS

TY - JOUR

T1 - Phage-Inducible Chromosomal Islands as a Diagnostic Platform to Capture and Detect Bacterial Pathogens

AU - Ibarra-Chávez, Rodrigo

AU - Reboud, Julien

AU - Penadés, José R

AU - Cooper, Jonathan M

PY - 2023

Y1 - 2023

N2 - Phage-inducible chromosomal islands (PICIs) are a family of phage satellites that hijack phage components to facilitate their mobility and spread. Recently, these genetic constructs are repurposed as antibacterial drones, enabling a new toolbox for unorthodox applications in biotechnology. To illustrate a new suite of functions, the authors have developed a user-friendly diagnostic system, based upon PICI transduction to selectively enrich bacteria, allowing the detection and sequential recovery of Escherichia coli and Staphylococcus aureus. The system enables high transfer rates and sensitivities in comparison with phages, with detection down to ≈50 CFU mL -1 . In contrast to conventional detection strategies, which often rely on nucleic acid molecular assays, and cannot differentiate between dead and live organisms, this approach enables visual sensing of viable pathogens only, through the expression of a reporter gene encoded in the PICI. The approach extends diagnostic sensing mechanisms beyond cell-free synthetic biology strategies, enabling new synthetic biology/biosensing toolkits.

AB - Phage-inducible chromosomal islands (PICIs) are a family of phage satellites that hijack phage components to facilitate their mobility and spread. Recently, these genetic constructs are repurposed as antibacterial drones, enabling a new toolbox for unorthodox applications in biotechnology. To illustrate a new suite of functions, the authors have developed a user-friendly diagnostic system, based upon PICI transduction to selectively enrich bacteria, allowing the detection and sequential recovery of Escherichia coli and Staphylococcus aureus. The system enables high transfer rates and sensitivities in comparison with phages, with detection down to ≈50 CFU mL -1 . In contrast to conventional detection strategies, which often rely on nucleic acid molecular assays, and cannot differentiate between dead and live organisms, this approach enables visual sensing of viable pathogens only, through the expression of a reporter gene encoded in the PICI. The approach extends diagnostic sensing mechanisms beyond cell-free synthetic biology strategies, enabling new synthetic biology/biosensing toolkits.

U2 - 10.1002/advs.202301643

DO - 10.1002/advs.202301643

M3 - Journal article

C2 - 37358000

VL - 10

JO - Advanced Science

JF - Advanced Science

SN - 2198-3844

IS - 24

M1 - 2301643

ER -

ID: 357780571

Department of Biology