Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging. / Trampe, Erik Christian Løvbjerg; Kolbowski, J.; Schreiber, U.; Kuhl, M.

In: Marine Biology, Vol. 158, No. 7, 2011, p. 1667-1675.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Trampe, ECL, Kolbowski, J, Schreiber, U & Kuhl, M 2011, 'Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging', Marine Biology, vol. 158, no. 7, pp. 1667-1675. https://doi.org/10.1007/s00227-011-1663-1

APA

Trampe, E. C. L., Kolbowski, J., Schreiber, U., & Kuhl, M. (2011). Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging. Marine Biology, 158(7), 1667-1675. https://doi.org/10.1007/s00227-011-1663-1

Vancouver

Trampe ECL, Kolbowski J, Schreiber U, Kuhl M. Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging. Marine Biology. 2011;158(7):1667-1675. https://doi.org/10.1007/s00227-011-1663-1

Author

Trampe, Erik Christian Løvbjerg ; Kolbowski, J. ; Schreiber, U. ; Kuhl, M. / Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging. In: Marine Biology. 2011 ; Vol. 158, No. 7. pp. 1667-1675.

Bibtex

@article{ab0af869c5144d948739d5f25fbf65ea,
title = "Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging",
abstract = "We present a new system for microscopic multicolour variable chlorophyll fluorescence imaging of aquatic phototrophs. The system is compact and portable and enables microscopic imaging of photosynthetic performance of individual cells and chloroplasts using different combinations of blue, green, red or white light. Automated sequential exposure of microscopic samples to the three excitation colours enables subsequent deconvolution of the resulting fluorescence signals and colour marking of cells with different photopigmentation, i.e., cyanobacteria, green algae, red algae and diatoms. The photosynthetic activity in complex mixtures of phototrophs and natural samples can thus be assigned to different types of phototrophs, which can be quantified simultaneously. Here, we describe the composition and performance of the new imaging system and present applications with both natural phytoplankton and microalgal culture samples",
keywords = "FAST REPETITION RATE, INDIVIDUAL ALGAL CELLS, A FLUORESCENCE, IN-VIVO, MICROBIAL COMMUNITIES, MARINE-PHYTOPLANKTON, LIGHT CURVES, PERFORMANCE, FLUOROMETRY, SEA",
author = "Trampe, {Erik Christian L{\o}vbjerg} and J. Kolbowski and U. Schreiber and M. Kuhl",
year = "2011",
doi = "10.1007/s00227-011-1663-1",
language = "English",
volume = "158",
pages = "1667--1675",
journal = "Marine Biology",
issn = "0025-3162",
publisher = "Springer",
number = "7",

}

RIS

TY - JOUR

T1 - Rapid assessment of different oxygenic phototrophs and single-cell photosynthesis with multicolour variable chlorophyll fluorescence imaging

AU - Trampe, Erik Christian Løvbjerg

AU - Kolbowski, J.

AU - Schreiber, U.

AU - Kuhl, M.

PY - 2011

Y1 - 2011

N2 - We present a new system for microscopic multicolour variable chlorophyll fluorescence imaging of aquatic phototrophs. The system is compact and portable and enables microscopic imaging of photosynthetic performance of individual cells and chloroplasts using different combinations of blue, green, red or white light. Automated sequential exposure of microscopic samples to the three excitation colours enables subsequent deconvolution of the resulting fluorescence signals and colour marking of cells with different photopigmentation, i.e., cyanobacteria, green algae, red algae and diatoms. The photosynthetic activity in complex mixtures of phototrophs and natural samples can thus be assigned to different types of phototrophs, which can be quantified simultaneously. Here, we describe the composition and performance of the new imaging system and present applications with both natural phytoplankton and microalgal culture samples

AB - We present a new system for microscopic multicolour variable chlorophyll fluorescence imaging of aquatic phototrophs. The system is compact and portable and enables microscopic imaging of photosynthetic performance of individual cells and chloroplasts using different combinations of blue, green, red or white light. Automated sequential exposure of microscopic samples to the three excitation colours enables subsequent deconvolution of the resulting fluorescence signals and colour marking of cells with different photopigmentation, i.e., cyanobacteria, green algae, red algae and diatoms. The photosynthetic activity in complex mixtures of phototrophs and natural samples can thus be assigned to different types of phototrophs, which can be quantified simultaneously. Here, we describe the composition and performance of the new imaging system and present applications with both natural phytoplankton and microalgal culture samples

KW - FAST REPETITION RATE

KW - INDIVIDUAL ALGAL CELLS

KW - A FLUORESCENCE

KW - IN-VIVO

KW - MICROBIAL COMMUNITIES

KW - MARINE-PHYTOPLANKTON

KW - LIGHT CURVES

KW - PERFORMANCE

KW - FLUOROMETRY

KW - SEA

U2 - 10.1007/s00227-011-1663-1

DO - 10.1007/s00227-011-1663-1

M3 - Journal article

VL - 158

SP - 1667

EP - 1675

JO - Marine Biology

JF - Marine Biology

SN - 0025-3162

IS - 7

ER -

ID: 37368127