Simultaneous visualization of flow fields and oxygen concentrations to unravel transport and metabolic processes in biological systems
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From individual cells to whole organisms, O-2 transport unfolds across micrometer- tomillimeter-length scales and can change within milliseconds in response to fluid flows and organismal behavior. The spatiotemporal complexity of these processes makes the accurate assessment of O-2 dynamics via currently availablemethods difficult or unreliable. Here, we present "sensPIV,'' a method to simultaneously measure O-2 concentrations and flow fields. By tracking O-2-sensitive microparticles in flow using imaging technologies that allow for instantaneous referencing, wemeasuredO(2) transportwithin (1) microfluidic devices, (2) sinkingmodel aggregates, and (3) complex colony-forming corals. Through the use of sensPIV, we find that corals use ciliarymovement to link zones of photosynthetic O-2 production to zones of O-2 consumption. SensPIV can potentially be extendable to study flow-organism interactions across many life-science and engineering applications.
Original language | English |
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Article number | 100216 |
Journal | Cell Reports Methods |
Volume | 2 |
Issue number | 5 |
Number of pages | 18 |
ISSN | 2667-2375 |
DOIs | |
Publication status | Published - 2022 |
- PARTICLE IMAGE VELOCIMETRY, O-2 DYNAMICS, FLUID, RHIZOSPHERE, VERSATILE, SEDIMENTS, FLUXES, ROOTS, CELLS, LIVE
Research areas
ID: 335675957