TY - JOUR

T1 - ß-adrenergic regulation of ion transport in pancreatic ducts: Patch-clamp study of isolated rat pancreatic ducts

AU - Novak, I

N1 - Keywords: Adenosine Triphosphate; Adrenergic beta-Agonists; Animals; Calcium; Carbachol; Electric Conductivity; Epinephrine; Female; Isoproterenol; Membrane Potentials; Pancreatic Ducts; Patch-Clamp Techniques; Phentolamine; Phenylephrine; Rats; Rats, Wistar; Receptors, Adrenergic, beta; Time Factors; Uridine Triphosphate

PY - 1998

Y1 - 1998

N2 - BACKGROUND & AIMS: In the intact pancreas, bicarbonate secretion is thought to be controlled by a number of regulators, including adrenergic agonists. The aim of this study was to investigate the effects of adrenergic agonists on pancreatic ducts, which are the site of bicarbonate secretion. METHODS: Small intralobular ducts were isolated from rat pancreas and studied in vitro by the whole-cell patch clamp technique. Cell membrane voltages and currents were indicators of cellular ion transport. In some ducts, intracellular Ca2+ activity was measured by fluorescence optical methods. RESULTS: Unstimulated duct cells had a membrane voltage (Vm) of about -50 mV. Isoproterenol had a concentration-dependent effect on Vm; at 10(-7) mol/L, it depolarized Vm by 20-25 mV and the cell conductance increased by 100 nanosiemens. These effects were a result of opening of luminal Cl- channels. Phenylephrine had much smaller effects. At comparable concentrations, it depolarized Vm by a few millivolts. Neither agonist had significant effects on intracellular Ca2+. CONCLUSIONS: This study provides the first direct evidence that adrenergic stimulation, namely, that of beta-adrenoceptors, controls ion transport in pancreatic ducts. Similar to secretin, isoproterenol stimulation leads to opening of luminal Cl- channels, and HCO3- enters the lumen in exchange for Cl-.

AB - BACKGROUND & AIMS: In the intact pancreas, bicarbonate secretion is thought to be controlled by a number of regulators, including adrenergic agonists. The aim of this study was to investigate the effects of adrenergic agonists on pancreatic ducts, which are the site of bicarbonate secretion. METHODS: Small intralobular ducts were isolated from rat pancreas and studied in vitro by the whole-cell patch clamp technique. Cell membrane voltages and currents were indicators of cellular ion transport. In some ducts, intracellular Ca2+ activity was measured by fluorescence optical methods. RESULTS: Unstimulated duct cells had a membrane voltage (Vm) of about -50 mV. Isoproterenol had a concentration-dependent effect on Vm; at 10(-7) mol/L, it depolarized Vm by 20-25 mV and the cell conductance increased by 100 nanosiemens. These effects were a result of opening of luminal Cl- channels. Phenylephrine had much smaller effects. At comparable concentrations, it depolarized Vm by a few millivolts. Neither agonist had significant effects on intracellular Ca2+. CONCLUSIONS: This study provides the first direct evidence that adrenergic stimulation, namely, that of beta-adrenoceptors, controls ion transport in pancreatic ducts. Similar to secretin, isoproterenol stimulation leads to opening of luminal Cl- channels, and HCO3- enters the lumen in exchange for Cl-.

M3 - Journal article

C2 - 9721169

VL - 115

SP - 714

EP - 721

JO - Gastroenterology

JF - Gastroenterology

SN - 0016-5085

IS - 3

ER -