Structural and Biophysical Properties of Supercharged and Circularized Nanodiscs
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Structural and Biophysical Properties of Supercharged and Circularized Nanodiscs. / Johansen, Nicolai Tidemand; Luchini, Alessandra; Tidemand, Frederik Grønbæk; Orioli, Simone; Martel, Anne; Porcar, Lionel; Arleth, Lise; Pedersen, Martin Cramer.
In: Langmuir, Vol. 37, No. 22, 2021, p. 6681-6690.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Structural and Biophysical Properties of Supercharged and Circularized Nanodiscs
AU - Johansen, Nicolai Tidemand
AU - Luchini, Alessandra
AU - Tidemand, Frederik Grønbæk
AU - Orioli, Simone
AU - Martel, Anne
AU - Porcar, Lionel
AU - Arleth, Lise
AU - Pedersen, Martin Cramer
N1 - Funding Information: The work was funded by the Lundbeck Foundation Brainstruc Programme (R155-2015-2666). Publisher Copyright: © 2021 American Chemical Society.
PY - 2021
Y1 - 2021
N2 - Nanodiscs based on membrane scaffold proteins (MSPs) and phospholipids are used as membrane mimics to stabilize membrane proteins in solution for structural and functional studies. Combining small-angle X-ray scattering (SAXS), differential scanning calorimetry (DSC), and time-resolved small-angle neutron scattering (TR-SANS), we characterized the structure and lipid bilayer properties of five different nanodiscs made with dimyristoylphosphatidylcholine and different MSPs varying in size, charge, and circularization. Our SAXS modeling showed that the structural parameters of the embedded lipids are all similar, irrespective of the MSP properties. DSC showed that the lipid packing is not homogeneous in the nanodiscs and that a 20 Å wide boundary layer of lipids with perturbed packing is located close to the MSP, while the packing of central lipids is tighter than in large unilamellar vesicles. Finally, TR-SANS showed that lipid exchange rates in nanodiscs decrease with increasing nanodisc size and are lower for the nanodiscs made with supercharged MSPs compared to conventional nanodiscs. Altogether, the results provide a thorough biophysical understanding of the nanodisc as a model membrane system, which is important in order to carry out and interpret experiments on membrane proteins embedded in such systems.
AB - Nanodiscs based on membrane scaffold proteins (MSPs) and phospholipids are used as membrane mimics to stabilize membrane proteins in solution for structural and functional studies. Combining small-angle X-ray scattering (SAXS), differential scanning calorimetry (DSC), and time-resolved small-angle neutron scattering (TR-SANS), we characterized the structure and lipid bilayer properties of five different nanodiscs made with dimyristoylphosphatidylcholine and different MSPs varying in size, charge, and circularization. Our SAXS modeling showed that the structural parameters of the embedded lipids are all similar, irrespective of the MSP properties. DSC showed that the lipid packing is not homogeneous in the nanodiscs and that a 20 Å wide boundary layer of lipids with perturbed packing is located close to the MSP, while the packing of central lipids is tighter than in large unilamellar vesicles. Finally, TR-SANS showed that lipid exchange rates in nanodiscs decrease with increasing nanodisc size and are lower for the nanodiscs made with supercharged MSPs compared to conventional nanodiscs. Altogether, the results provide a thorough biophysical understanding of the nanodisc as a model membrane system, which is important in order to carry out and interpret experiments on membrane proteins embedded in such systems.
U2 - 10.1021/acs.langmuir.1c00560
DO - 10.1021/acs.langmuir.1c00560
M3 - Journal article
C2 - 34038130
AN - SCOPUS:85108021041
VL - 37
SP - 6681
EP - 6690
JO - Langmuir
JF - Langmuir
SN - 0743-7463
IS - 22
ER -
ID: 274115184