The cyclin-dependent kinase 8 module sterically blocks Mediator interactions with RNA polymerase II
Research output: Contribution to journal › Journal article › Research › peer-review
CDK8 (cyclin-dependent kinase 8), along with CycC, Med12, and Med13, form a repressive module (the Cdk8 module) that prevents RNA polymerase II (pol II) interactions with Mediator. Here, we report that the ability of the Cdk8 module to prevent pol II interactions is independent of the Cdk8-dependent kinase activity. We use electron microscopy and single-particle reconstruction to demonstrate that the Cdk8 module forms a distinct structural entity that binds to the head and middle region of Mediator, thereby sterically blocking interactions with pol II.
| Original language | English |
|---|---|
| Journal | Proceedings of the National Academy of Science of the United States of America |
| Volume | 103 |
| Issue number | 43 |
| Pages (from-to) | 15788-93 |
| Number of pages | 5 |
| ISSN | 0027-8424 |
| DOIs | |
| Publication status | Published - 2006 |
Bibliographical note
Keywords: Cyclin-Dependent Kinases; Holoenzymes; Microscopy, Electron; Models, Molecular; Protein Binding; Protein Structure, Quaternary; Protein Structure, Tertiary; RNA Polymerase II; Schizosaccharomyces; Schizosaccharomyces pombe Proteins
ID: 9250702