The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of cystic fibrosis patients
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The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of cystic fibrosis patients. / Holden, Matthew T G; Seth-Smith, Helena M B; Crossman, Lisa C; Sebaihia, Mohammed; Bentley, Stephen D; Cerdeño-Tárraga, Ana M; Thomson, Nicholas R; Bason, Nathalie; Quail, Michael A; Sharp, Sarah; Cherevach, Inna; Churcher, Carol; Goodhead, Ian; Hauser, Heidi; Holroyd, Nancy; Mungall, Karen; Scott, Paul; Walker, Danielle; White, Brian; Rose, Helen; Iversen, Pernille; Mil-Homens, Dalila; Rocha, Eduardo P C; Fialho, Arsenio M; Baldwin, Adam; Dowson, Christopher; Barrell, Bart G; Govan, John R; Vandamme, Peter; Hart, C Anthony; Mahenthiralingam, Eshwar; Parkhill, Julian.
In: Journal of Bacteriology, Vol. 191, No. 1, 2009, p. 261-77.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The genome of Burkholderia cenocepacia J2315, an epidemic pathogen of cystic fibrosis patients
AU - Holden, Matthew T G
AU - Seth-Smith, Helena M B
AU - Crossman, Lisa C
AU - Sebaihia, Mohammed
AU - Bentley, Stephen D
AU - Cerdeño-Tárraga, Ana M
AU - Thomson, Nicholas R
AU - Bason, Nathalie
AU - Quail, Michael A
AU - Sharp, Sarah
AU - Cherevach, Inna
AU - Churcher, Carol
AU - Goodhead, Ian
AU - Hauser, Heidi
AU - Holroyd, Nancy
AU - Mungall, Karen
AU - Scott, Paul
AU - Walker, Danielle
AU - White, Brian
AU - Rose, Helen
AU - Iversen, Pernille
AU - Mil-Homens, Dalila
AU - Rocha, Eduardo P C
AU - Fialho, Arsenio M
AU - Baldwin, Adam
AU - Dowson, Christopher
AU - Barrell, Bart G
AU - Govan, John R
AU - Vandamme, Peter
AU - Hart, C Anthony
AU - Mahenthiralingam, Eshwar
AU - Parkhill, Julian
PY - 2009
Y1 - 2009
N2 - Bacterial infections of the lungs of cystic fibrosis (CF) patients cause major complications in the treatment of this common genetic disease. Burkholderia cenocepacia infection is particularly problematic since this organism has high levels of antibiotic resistance, making it difficult to eradicate; the resulting chronic infections are associated with severe declines in lung function and increased mortality rates. B. cenocepacia strain J2315 was isolated from a CF patient and is a member of the epidemic ET12 lineage that originated in Canada or the United Kingdom and spread to Europe. The 8.06-Mb genome of this highly transmissible pathogen comprises three circular chromosomes and a plasmid and encodes a broad array of functions typical of this metabolically versatile genus, as well as numerous virulence and drug resistance functions. Although B. cenocepacia strains can be isolated from soil and can be pathogenic to both plants and man, J2315 is representative of a lineage of B. cenocepacia rarely isolated from the environment and which spreads between CF patients. Comparative analysis revealed that ca. 21% of the genome is unique in comparison to other strains of B. cenocepacia, highlighting the genomic plasticity of this species. Pseudogenes in virulence determinants suggest that the pathogenic response of J2315 may have been recently selected to promote persistence in the CF lung. The J2315 genome contains evidence that its unique and highly adapted genetic content has played a significant role in its success as an epidemic CF pathogen.
AB - Bacterial infections of the lungs of cystic fibrosis (CF) patients cause major complications in the treatment of this common genetic disease. Burkholderia cenocepacia infection is particularly problematic since this organism has high levels of antibiotic resistance, making it difficult to eradicate; the resulting chronic infections are associated with severe declines in lung function and increased mortality rates. B. cenocepacia strain J2315 was isolated from a CF patient and is a member of the epidemic ET12 lineage that originated in Canada or the United Kingdom and spread to Europe. The 8.06-Mb genome of this highly transmissible pathogen comprises three circular chromosomes and a plasmid and encodes a broad array of functions typical of this metabolically versatile genus, as well as numerous virulence and drug resistance functions. Although B. cenocepacia strains can be isolated from soil and can be pathogenic to both plants and man, J2315 is representative of a lineage of B. cenocepacia rarely isolated from the environment and which spreads between CF patients. Comparative analysis revealed that ca. 21% of the genome is unique in comparison to other strains of B. cenocepacia, highlighting the genomic plasticity of this species. Pseudogenes in virulence determinants suggest that the pathogenic response of J2315 may have been recently selected to promote persistence in the CF lung. The J2315 genome contains evidence that its unique and highly adapted genetic content has played a significant role in its success as an epidemic CF pathogen.
U2 - 10.1128/JB.01230-08
DO - 10.1128/JB.01230-08
M3 - Journal article
C2 - 18931103
VL - 191
SP - 261
EP - 277
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 1
ER -
ID: 9542140