The genome of Magnolia biondii Pamp. provides insights into the evolution of Magnoliales and biosynthesis of terpenoids

Research output: Contribution to journal › Journal article › Research › peer-review

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The genome of Magnolia biondii Pamp. provides insights into the evolution of Magnoliales and biosynthesis of terpenoids. / Dong, Shanshan; Liu, Min; Liu, Yang; Chen, Fei; Yang, Ting; Chen, Lu; Zhang, Xingtan; Guo, Xing; Fang, Dongming; Li, Linzhou; Deng, Tian; Yao, Zhangxiu; Lang, Xiaoan; Gong, Yiqing; Wu, Ernest; Wang, Yaling; Shen, Yamei; Gong, Xun; Liu, Huan; Zhang, Shouzhou.

In: Horticulture Research, Vol. 8, 38, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Dong, S, Liu, M, Liu, Y, Chen, F, Yang, T, Chen, L, Zhang, X, Guo, X, Fang, D, Li, L, Deng, T, Yao, Z, Lang, X, Gong, Y, Wu, E, Wang, Y, Shen, Y, Gong, X, Liu, H & Zhang, S 2021, 'The genome of Magnolia biondii Pamp. provides insights into the evolution of Magnoliales and biosynthesis of terpenoids', Horticulture Research, vol. 8, 38. https://doi.org/10.1038/s41438-021-00471-9

APA

Dong, S., Liu, M., Liu, Y., Chen, F., Yang, T., Chen, L., Zhang, X., Guo, X., Fang, D., Li, L., Deng, T., Yao, Z., Lang, X., Gong, Y., Wu, E., Wang, Y., Shen, Y., Gong, X., Liu, H., & Zhang, S. (2021). The genome of Magnolia biondii Pamp. provides insights into the evolution of Magnoliales and biosynthesis of terpenoids. Horticulture Research, 8, [38]. https://doi.org/10.1038/s41438-021-00471-9

Vancouver

Dong S, Liu M, Liu Y, Chen F, Yang T, Chen L et al. The genome of Magnolia biondii Pamp. provides insights into the evolution of Magnoliales and biosynthesis of terpenoids. Horticulture Research. 2021;8. 38. https://doi.org/10.1038/s41438-021-00471-9

Author

Dong, Shanshan ; Liu, Min ; Liu, Yang ; Chen, Fei ; Yang, Ting ; Chen, Lu ; Zhang, Xingtan ; Guo, Xing ; Fang, Dongming ; Li, Linzhou ; Deng, Tian ; Yao, Zhangxiu ; Lang, Xiaoan ; Gong, Yiqing ; Wu, Ernest ; Wang, Yaling ; Shen, Yamei ; Gong, Xun ; Liu, Huan ; Zhang, Shouzhou. / The genome of Magnolia biondii Pamp. provides insights into the evolution of Magnoliales and biosynthesis of terpenoids. In: Horticulture Research. 2021 ; Vol. 8.

Bibtex

@article{fdc9c62afb8a45dfbb747dd149d854d5,
title = "The genome of Magnolia biondii Pamp. provides insights into the evolution of Magnoliales and biosynthesis of terpenoids",
abstract = "Magnolia biondii Pamp. (Magnoliaceae, magnoliids) is a phylogenetically, economically, and medicinally important ornamental tree species widely grown and cultivated in the north-temperate regions of China. Determining the genome sequence of M. biondii would help resolve the phylogenetic uncertainty of magnoliids and improve the understanding of individual trait evolution within the Magnolia genus. We assembled a chromosome-level reference genome of M. biondii using ~67, ~175, and ~154 Gb of raw DNA sequences generated via Pacific Biosciences single-molecule real-time sequencing, 10X Genomics Chromium, and Hi-C scaffolding strategies, respectively. The final genome assembly was ~2.22 Gb, with a contig N50 value of 269.11 kb and a BUSCO complete gene percentage of 91.90%. Approximately 89.17% of the genome was organized into 19 chromosomes, resulting in a scaffold N50 of 92.86 Mb. The genome contained 47,547 protein-coding genes, accounting for 23.47% of the genome length, whereas 66.48% of the genome length consisted of repetitive elements. We confirmed a WGD event that occurred very close to the time of the split between the Magnoliales and Laurales. Functional enrichment of the Magnolia-specific and expanded gene families highlighted genes involved in the biosynthesis of secondary metabolites, plant–pathogen interactions, and responses to stimuli, which may improve the ecological fitness and biological adaptability of the lineage. Phylogenomic analyses revealed a sister relationship of magnoliids and Chloranthaceae, which are sister to a clade comprising monocots and eudicots. The genome sequence of M. biondii could lead to trait improvement, germplasm conservation, and evolutionary studies on the rapid radiation of early angiosperms.",
author = "Shanshan Dong and Min Liu and Yang Liu and Fei Chen and Ting Yang and Lu Chen and Xingtan Zhang and Xing Guo and Dongming Fang and Linzhou Li and Tian Deng and Zhangxiu Yao and Xiaoan Lang and Yiqing Gong and Ernest Wu and Yaling Wang and Yamei Shen and Xun Gong and Huan Liu and Shouzhou Zhang",
year = "2021",
doi = "10.1038/s41438-021-00471-9",
language = "English",
volume = "8",
journal = "Horticulture Research",
issn = "2662-6810",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - The genome of Magnolia biondii Pamp. provides insights into the evolution of Magnoliales and biosynthesis of terpenoids

AU - Dong, Shanshan

AU - Liu, Min

AU - Liu, Yang

AU - Chen, Fei

AU - Yang, Ting

AU - Chen, Lu

AU - Zhang, Xingtan

AU - Guo, Xing

AU - Fang, Dongming

AU - Li, Linzhou

AU - Deng, Tian

AU - Yao, Zhangxiu

AU - Lang, Xiaoan

AU - Gong, Yiqing

AU - Wu, Ernest

AU - Wang, Yaling

AU - Shen, Yamei

AU - Gong, Xun

AU - Liu, Huan

AU - Zhang, Shouzhou

PY - 2021

Y1 - 2021

N2 - Magnolia biondii Pamp. (Magnoliaceae, magnoliids) is a phylogenetically, economically, and medicinally important ornamental tree species widely grown and cultivated in the north-temperate regions of China. Determining the genome sequence of M. biondii would help resolve the phylogenetic uncertainty of magnoliids and improve the understanding of individual trait evolution within the Magnolia genus. We assembled a chromosome-level reference genome of M. biondii using ~67, ~175, and ~154 Gb of raw DNA sequences generated via Pacific Biosciences single-molecule real-time sequencing, 10X Genomics Chromium, and Hi-C scaffolding strategies, respectively. The final genome assembly was ~2.22 Gb, with a contig N50 value of 269.11 kb and a BUSCO complete gene percentage of 91.90%. Approximately 89.17% of the genome was organized into 19 chromosomes, resulting in a scaffold N50 of 92.86 Mb. The genome contained 47,547 protein-coding genes, accounting for 23.47% of the genome length, whereas 66.48% of the genome length consisted of repetitive elements. We confirmed a WGD event that occurred very close to the time of the split between the Magnoliales and Laurales. Functional enrichment of the Magnolia-specific and expanded gene families highlighted genes involved in the biosynthesis of secondary metabolites, plant–pathogen interactions, and responses to stimuli, which may improve the ecological fitness and biological adaptability of the lineage. Phylogenomic analyses revealed a sister relationship of magnoliids and Chloranthaceae, which are sister to a clade comprising monocots and eudicots. The genome sequence of M. biondii could lead to trait improvement, germplasm conservation, and evolutionary studies on the rapid radiation of early angiosperms.

AB - Magnolia biondii Pamp. (Magnoliaceae, magnoliids) is a phylogenetically, economically, and medicinally important ornamental tree species widely grown and cultivated in the north-temperate regions of China. Determining the genome sequence of M. biondii would help resolve the phylogenetic uncertainty of magnoliids and improve the understanding of individual trait evolution within the Magnolia genus. We assembled a chromosome-level reference genome of M. biondii using ~67, ~175, and ~154 Gb of raw DNA sequences generated via Pacific Biosciences single-molecule real-time sequencing, 10X Genomics Chromium, and Hi-C scaffolding strategies, respectively. The final genome assembly was ~2.22 Gb, with a contig N50 value of 269.11 kb and a BUSCO complete gene percentage of 91.90%. Approximately 89.17% of the genome was organized into 19 chromosomes, resulting in a scaffold N50 of 92.86 Mb. The genome contained 47,547 protein-coding genes, accounting for 23.47% of the genome length, whereas 66.48% of the genome length consisted of repetitive elements. We confirmed a WGD event that occurred very close to the time of the split between the Magnoliales and Laurales. Functional enrichment of the Magnolia-specific and expanded gene families highlighted genes involved in the biosynthesis of secondary metabolites, plant–pathogen interactions, and responses to stimuli, which may improve the ecological fitness and biological adaptability of the lineage. Phylogenomic analyses revealed a sister relationship of magnoliids and Chloranthaceae, which are sister to a clade comprising monocots and eudicots. The genome sequence of M. biondii could lead to trait improvement, germplasm conservation, and evolutionary studies on the rapid radiation of early angiosperms.

U2 - 10.1038/s41438-021-00471-9

DO - 10.1038/s41438-021-00471-9

M3 - Journal article

C2 - 33642574

AN - SCOPUS:85101818156

VL - 8

JO - Horticulture Research

JF - Horticulture Research

SN - 2662-6810

M1 - 38

ER -

ID: 260186204