The immune privilege of the eye: human retinal pigment epithelial cells selectively modulate T-cell activation in vitro.
Research output: Contribution to journal › Journal article › Research › peer-review
PURPOSE: To examine the effect of human retinal pigment epithelial (RPE) cells on phytohemagglutinin (PHA) activation of T cells. METHODS: Resting peripheral blood lymphocytes (PBLs) were stimulated with PHA with or without the presence of gamma-irradiated RPE cells. Proliferation and the cell cycle profile were thereafter investigated by 3H-thymidine incorporation and flow cytometric analysis. In addition, the PBLs expression of CD69, major histocompatibility complex (MHC) class I and II, CD3, as well as the IL-2 receptor chains were evaluated by flow cytometry, and the content of IL-2 in cell culture supernatant was measured by ELISA. RESULTS: Human RPE cells were found to suppress PHA-induced proliferation, cyclin A, IL-2R-alpha and -gamma, and CD71 expression and decrease the production of IL-2; but RPE cells do not inhibit the PHA-induced expression of early activation markers CD69, MHC class I and II, and of cyclin D of the PBLs. CONCLUSIONS: These results are the first to indicate that RPE cells impede generation of activated T cells by interfering with the induction of high-affinity IL-2R-alphabetagamma, IL-2 production, and the expression of CD71 and cyclin A.
Original language | English |
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Journal | Current Eye Research |
Volume | 30 |
Issue number | 5 |
Pages (from-to) | 375-83 |
Number of pages | 8 |
ISSN | 0271-3683 |
DOIs | |
Publication status | Published - 2005 |
Bibliographical note
Keywords: Antigens, CD; Antigens, CD3; Antigens, Differentiation, T-Lymphocyte; Cell Cycle; Cell Line; Cell Proliferation; Coculture Techniques; DNA Replication; Enzyme-Linked Immunosorbent Assay; Eye; Flow Cytometry; Histocompatibility Antigens Class I; Histocompatibility Antigens Class II; Humans; Immune System; Interleukin-2 Receptor alpha Subunit; Lymphocyte Activation; Pigment Epithelium of Eye; Receptors, Interleukin; T-Lymphocytes
ID: 8724898