The LEAFY floral regulator displays pioneer transcription factor properties
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The LEAFY floral regulator displays pioneer transcription factor properties. / Lai, Xuelei; Blanc-Mathieu, Romain; GrandVuillemin, Loïc; Huang, Ying; Stigliani, Arnaud; Lucas, Jérémy; Thévenon, Emmanuel; Loue-Manifel, Jeanne; Turchi, Laura; Daher, Hussein; Brun-Hernandez, Eugenia; Vachon, Gilles; Latrasse, David; Benhamed, Moussa; Dumas, Renaud; Zubieta, Chloe; Parcy, François.
In: Molecular Plant, Vol. 14, No. 5, 2021, p. 829-837.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The LEAFY floral regulator displays pioneer transcription factor properties
AU - Lai, Xuelei
AU - Blanc-Mathieu, Romain
AU - GrandVuillemin, Loïc
AU - Huang, Ying
AU - Stigliani, Arnaud
AU - Lucas, Jérémy
AU - Thévenon, Emmanuel
AU - Loue-Manifel, Jeanne
AU - Turchi, Laura
AU - Daher, Hussein
AU - Brun-Hernandez, Eugenia
AU - Vachon, Gilles
AU - Latrasse, David
AU - Benhamed, Moussa
AU - Dumas, Renaud
AU - Zubieta, Chloe
AU - Parcy, François
N1 - Publisher Copyright: © 2021 The Author
PY - 2021
Y1 - 2021
N2 - Pioneer transcription factors (TFs) are a special category of TFs with the capacity to bind to closed chromatin regions in which DNA is wrapped around histones and may be highly methylated. Subsequently, pioneer TFs are able to modify the chromatin state to initiate gene expression. In plants, LEAFY (LFY) is a master floral regulator and has been suggested to act as a pioneer TF in Arabidopsis. Here, we demonstrate that LFY is able to bind both methylated and non-methylated DNA using a combination of in vitro genome-wide binding experiments and structural modeling. Comparisons between regions bound by LFY in vivo and chromatin accessibility data suggest that a subset of LFY bound regions is occupied by nucleosomes. We confirm that LFY is able to bind nucleosomal DNA in vitro using reconstituted nucleosomes. Finally, we show that constitutive LFY expression in seedling tissues is sufficient to induce chromatin accessibility in the LFY direct target genes APETALA1 and AGAMOUS. Taken together, our study suggests that LFY possesses key pioneer TF features that contribute to launching the floral gene expression program.
AB - Pioneer transcription factors (TFs) are a special category of TFs with the capacity to bind to closed chromatin regions in which DNA is wrapped around histones and may be highly methylated. Subsequently, pioneer TFs are able to modify the chromatin state to initiate gene expression. In plants, LEAFY (LFY) is a master floral regulator and has been suggested to act as a pioneer TF in Arabidopsis. Here, we demonstrate that LFY is able to bind both methylated and non-methylated DNA using a combination of in vitro genome-wide binding experiments and structural modeling. Comparisons between regions bound by LFY in vivo and chromatin accessibility data suggest that a subset of LFY bound regions is occupied by nucleosomes. We confirm that LFY is able to bind nucleosomal DNA in vitro using reconstituted nucleosomes. Finally, we show that constitutive LFY expression in seedling tissues is sufficient to induce chromatin accessibility in the LFY direct target genes APETALA1 and AGAMOUS. Taken together, our study suggests that LFY possesses key pioneer TF features that contribute to launching the floral gene expression program.
KW - chromatin remodeling
KW - flower development
KW - gene regulation
KW - pioneer transcription factor
U2 - 10.1016/j.molp.2021.03.004
DO - 10.1016/j.molp.2021.03.004
M3 - Journal article
C2 - 33684542
AN - SCOPUS:85103522424
VL - 14
SP - 829
EP - 837
JO - Molecular Plant
JF - Molecular Plant
SN - 1674-2052
IS - 5
ER -
ID: 262845051