The rapid “teabag” method for high-end purification of membrane proteins

Research output: Contribution to journal › Journal article › Research › peer-review

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The rapid “teabag” method for high-end purification of membrane proteins. / Hering, Jenny; Missel, Julie Winkel; Zhang, Liying; Gunnarsson, Anders; Castaldo, Marie; Pedersen, Per Amstrup; Ek, Margareta; Gourdon, Pontus; Snijder, Harm Jan.

In: Scientific Reports, Vol. 10, 16167, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hering, J, Missel, JW, Zhang, L, Gunnarsson, A, Castaldo, M, Pedersen, PA, Ek, M, Gourdon, P & Snijder, HJ 2020, 'The rapid “teabag” method for high-end purification of membrane proteins', Scientific Reports, vol. 10, 16167. https://doi.org/10.1038/s41598-020-73285-9

APA

Hering, J., Missel, J. W., Zhang, L., Gunnarsson, A., Castaldo, M., Pedersen, P. A., Ek, M., Gourdon, P., & Snijder, H. J. (2020). The rapid “teabag” method for high-end purification of membrane proteins. Scientific Reports, 10, [16167]. https://doi.org/10.1038/s41598-020-73285-9

Vancouver

Hering J, Missel JW, Zhang L, Gunnarsson A, Castaldo M, Pedersen PA et al. The rapid “teabag” method for high-end purification of membrane proteins. Scientific Reports. 2020;10. 16167. https://doi.org/10.1038/s41598-020-73285-9

Author

Hering, Jenny ; Missel, Julie Winkel ; Zhang, Liying ; Gunnarsson, Anders ; Castaldo, Marie ; Pedersen, Per Amstrup ; Ek, Margareta ; Gourdon, Pontus ; Snijder, Harm Jan. / The rapid “teabag” method for high-end purification of membrane proteins. In: Scientific Reports. 2020 ; Vol. 10.

Bibtex

@article{4814e3efe9534d24b891b1a48e46af88,
title = "The rapid “teabag” method for high-end purification of membrane proteins",
abstract = "Overproduction and purification of membrane proteins are generally challenging and time-consuming procedures due to low expression levels, misfolding, and low stability once extracted from the membrane. Reducing processing steps and shortening the timespan for purification represent attractive approaches to overcome some of these challenges. We have therefore compared a fast “teabag” purification method with conventional purification for five different membrane proteins (MraY, AQP10, ClC-1, PAR2 and KCC2). Notably, this new approach reduces the purification time significantly, and the quality of the purified membrane proteins is equal to or exceeds conventional methods as assessed by size exclusion chromatography, SDS-PAGE and downstream applications such as ITC, crystallization and cryo-EM. Furthermore, the method is scalable, applicable to a range of affinity resins and allows for parallelization. Consequently, the technique has the potential to substantially simplify purification efforts of membrane proteins in basic and applied sciences.",
author = "Jenny Hering and Missel, {Julie Winkel} and Liying Zhang and Anders Gunnarsson and Marie Castaldo and Pedersen, {Per Amstrup} and Margareta Ek and Pontus Gourdon and Snijder, {Harm Jan}",
year = "2020",
doi = "10.1038/s41598-020-73285-9",
language = "English",
volume = "10",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - The rapid “teabag” method for high-end purification of membrane proteins

AU - Hering, Jenny

AU - Missel, Julie Winkel

AU - Zhang, Liying

AU - Gunnarsson, Anders

AU - Castaldo, Marie

AU - Pedersen, Per Amstrup

AU - Ek, Margareta

AU - Gourdon, Pontus

AU - Snijder, Harm Jan

PY - 2020

Y1 - 2020

N2 - Overproduction and purification of membrane proteins are generally challenging and time-consuming procedures due to low expression levels, misfolding, and low stability once extracted from the membrane. Reducing processing steps and shortening the timespan for purification represent attractive approaches to overcome some of these challenges. We have therefore compared a fast “teabag” purification method with conventional purification for five different membrane proteins (MraY, AQP10, ClC-1, PAR2 and KCC2). Notably, this new approach reduces the purification time significantly, and the quality of the purified membrane proteins is equal to or exceeds conventional methods as assessed by size exclusion chromatography, SDS-PAGE and downstream applications such as ITC, crystallization and cryo-EM. Furthermore, the method is scalable, applicable to a range of affinity resins and allows for parallelization. Consequently, the technique has the potential to substantially simplify purification efforts of membrane proteins in basic and applied sciences.

AB - Overproduction and purification of membrane proteins are generally challenging and time-consuming procedures due to low expression levels, misfolding, and low stability once extracted from the membrane. Reducing processing steps and shortening the timespan for purification represent attractive approaches to overcome some of these challenges. We have therefore compared a fast “teabag” purification method with conventional purification for five different membrane proteins (MraY, AQP10, ClC-1, PAR2 and KCC2). Notably, this new approach reduces the purification time significantly, and the quality of the purified membrane proteins is equal to or exceeds conventional methods as assessed by size exclusion chromatography, SDS-PAGE and downstream applications such as ITC, crystallization and cryo-EM. Furthermore, the method is scalable, applicable to a range of affinity resins and allows for parallelization. Consequently, the technique has the potential to substantially simplify purification efforts of membrane proteins in basic and applied sciences.

U2 - 10.1038/s41598-020-73285-9

DO - 10.1038/s41598-020-73285-9

M3 - Journal article

C2 - 32999380

AN - SCOPUS:85091717292

VL - 10

JO - Scientific Reports

JF - Scientific Reports

SN - 2045-2322

M1 - 16167

ER -

ID: 250543975