The Voltage-Gated Sodium Channel Beta4 Subunit Maintains Epithelial Phenotype in Mammary Cells

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The Voltage-Gated Sodium Channel Beta4 Subunit Maintains Epithelial Phenotype in Mammary Cells. / Doray, Adélaïde; Lemoine, Roxane; Severin, Marc; Chadet, Stéphanie; Lopez-Charcas, Osbaldo; Héraud, Audrey; Baron, Christophe; Besson, Pierre; Monteil, Arnaud; Pedersen, Stine Falsig; Roger, Sébastien.

In: Cells, Vol. 10, No. 7, 1624, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Doray, A, Lemoine, R, Severin, M, Chadet, S, Lopez-Charcas, O, Héraud, A, Baron, C, Besson, P, Monteil, A, Pedersen, SF & Roger, S 2021, 'The Voltage-Gated Sodium Channel Beta4 Subunit Maintains Epithelial Phenotype in Mammary Cells', Cells, vol. 10, no. 7, 1624. https://doi.org/10.3390/cells10071624

APA

Doray, A., Lemoine, R., Severin, M., Chadet, S., Lopez-Charcas, O., Héraud, A., Baron, C., Besson, P., Monteil, A., Pedersen, S. F., & Roger, S. (2021). The Voltage-Gated Sodium Channel Beta4 Subunit Maintains Epithelial Phenotype in Mammary Cells. Cells, 10(7), [1624]. https://doi.org/10.3390/cells10071624

Vancouver

Doray A, Lemoine R, Severin M, Chadet S, Lopez-Charcas O, Héraud A et al. The Voltage-Gated Sodium Channel Beta4 Subunit Maintains Epithelial Phenotype in Mammary Cells. Cells. 2021;10(7). 1624. https://doi.org/10.3390/cells10071624

Author

Doray, Adélaïde ; Lemoine, Roxane ; Severin, Marc ; Chadet, Stéphanie ; Lopez-Charcas, Osbaldo ; Héraud, Audrey ; Baron, Christophe ; Besson, Pierre ; Monteil, Arnaud ; Pedersen, Stine Falsig ; Roger, Sébastien. / The Voltage-Gated Sodium Channel Beta4 Subunit Maintains Epithelial Phenotype in Mammary Cells. In: Cells. 2021 ; Vol. 10, No. 7.

Bibtex

@article{e1469cdc349a48c98c4fe1985d94634d,
title = "The Voltage-Gated Sodium Channel Beta4 Subunit Maintains Epithelial Phenotype in Mammary Cells",
abstract = "The SCN4B gene, coding for the NaVβ4 subunit of voltage-gated sodium channels, was recently found to be expressed in normal epithelial cells and down-regulated in several cancers. However, its function in normal epithelial cells has not been characterized. In this study, we demonstrated that reducing NaVβ4 expression in MCF10A non-cancer mammary epithelial cells generated important morphological changes observed both in two-dimensional cultures and in three-dimensional cysts. Most notably, the loss of NaVβ4 induced a complete loss of epithelial organisation in cysts and increased proteolytic activity towards the extracellular matrix. Loss of epithelial morphology was associated with an increased degradation of β-catenin, reduced E-cadherin expression and induction of mesenchymal markers N-cadherin, vimentin, and α-SMA expression. Overall, our results suggest that Navβ4 may participate in the maintenance of the epithelial phenotype in mammary cells and that its downregulation might be a determining step in early carcinogenesis.",
keywords = "epithelial phenotype, epithelial-to-mesenchymal transition, mammary cells, NaVβ4, β-catenin",
author = "Ad{\'e}la{\"i}de Doray and Roxane Lemoine and Marc Severin and St{\'e}phanie Chadet and Osbaldo Lopez-Charcas and Audrey H{\'e}raud and Christophe Baron and Pierre Besson and Arnaud Monteil and Pedersen, {Stine Falsig} and S{\'e}bastien Roger",
year = "2021",
doi = "10.3390/cells10071624",
language = "English",
volume = "10",
journal = "Cells",
issn = "2073-4409",
publisher = "MDPI AG",
number = "7",

}

RIS

TY - JOUR

T1 - The Voltage-Gated Sodium Channel Beta4 Subunit Maintains Epithelial Phenotype in Mammary Cells

AU - Doray, Adélaïde

AU - Lemoine, Roxane

AU - Severin, Marc

AU - Chadet, Stéphanie

AU - Lopez-Charcas, Osbaldo

AU - Héraud, Audrey

AU - Baron, Christophe

AU - Besson, Pierre

AU - Monteil, Arnaud

AU - Pedersen, Stine Falsig

AU - Roger, Sébastien

PY - 2021

Y1 - 2021

N2 - The SCN4B gene, coding for the NaVβ4 subunit of voltage-gated sodium channels, was recently found to be expressed in normal epithelial cells and down-regulated in several cancers. However, its function in normal epithelial cells has not been characterized. In this study, we demonstrated that reducing NaVβ4 expression in MCF10A non-cancer mammary epithelial cells generated important morphological changes observed both in two-dimensional cultures and in three-dimensional cysts. Most notably, the loss of NaVβ4 induced a complete loss of epithelial organisation in cysts and increased proteolytic activity towards the extracellular matrix. Loss of epithelial morphology was associated with an increased degradation of β-catenin, reduced E-cadherin expression and induction of mesenchymal markers N-cadherin, vimentin, and α-SMA expression. Overall, our results suggest that Navβ4 may participate in the maintenance of the epithelial phenotype in mammary cells and that its downregulation might be a determining step in early carcinogenesis.

AB - The SCN4B gene, coding for the NaVβ4 subunit of voltage-gated sodium channels, was recently found to be expressed in normal epithelial cells and down-regulated in several cancers. However, its function in normal epithelial cells has not been characterized. In this study, we demonstrated that reducing NaVβ4 expression in MCF10A non-cancer mammary epithelial cells generated important morphological changes observed both in two-dimensional cultures and in three-dimensional cysts. Most notably, the loss of NaVβ4 induced a complete loss of epithelial organisation in cysts and increased proteolytic activity towards the extracellular matrix. Loss of epithelial morphology was associated with an increased degradation of β-catenin, reduced E-cadherin expression and induction of mesenchymal markers N-cadherin, vimentin, and α-SMA expression. Overall, our results suggest that Navβ4 may participate in the maintenance of the epithelial phenotype in mammary cells and that its downregulation might be a determining step in early carcinogenesis.

KW - epithelial phenotype

KW - epithelial-to-mesenchymal transition

KW - mammary cells

KW - NaVβ4

KW - β-catenin

U2 - 10.3390/cells10071624

DO - 10.3390/cells10071624

M3 - Journal article

C2 - 34209614

AN - SCOPUS:85110343856

VL - 10

JO - Cells

JF - Cells

SN - 2073-4409

IS - 7

M1 - 1624

ER -

ID: 275880689