Typing for HLA-DPB1*03 and HLA-DPB1*06 using allele-specific DNA in vitro amplification and allele-specific oligonucleotide probes. Detection of "new" DPB1*06 variants
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Typing for HLA-DPB1*03 and HLA-DPB1*06 using allele-specific DNA in vitro amplification and allele-specific oligonucleotide probes. Detection of "new" DPB1*06 variants. / Fugger, L; Morling, N; Ryder, L P; Ødum, Niels; Georgsen, J; Svejgaard, A.
In: Immunogenetics, Vol. 30, No. 3, 1989, p. 208-13.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Typing for HLA-DPB1*03 and HLA-DPB1*06 using allele-specific DNA in vitro amplification and allele-specific oligonucleotide probes. Detection of "new" DPB1*06 variants
AU - Fugger, L
AU - Morling, N
AU - Ryder, L P
AU - Ødum, Niels
AU - Georgsen, J
AU - Svejgaard, A
N1 - Keywords: Alleles; Amino Acid Sequence; Base Sequence; Gene Amplification; HLA-DP Antigens; Humans; Molecular Sequence Data; Nucleic Acid Hybridization; Oligonucleotide Probes; Polymorphism, Genetic
PY - 1989
Y1 - 1989
N2 - DP gene typing using in vitro DNA amplification combined with sequence-specific oligonucleotide probes has recently been reported. The resulting DNA amplification was specific for the HLA-DPB locus. Typing for the individual DPB alleles was exclusively dependent on the hybridizations of the probes but hampered by close sequence homology between different DP alleles yielding complex patterns of reactivity with a panel of probes. We report the combined use of allele-specific DNA in vitro amplification and allele-specific oligonucleotides in typing for DPB1*03 and DPB1*06. Complete concordance with PLT typing was observed for the DPB1*03 alleles, while in the DPB1*06 group, at least three variant DPB1*06 alleles were identified which have not been described previously.
AB - DP gene typing using in vitro DNA amplification combined with sequence-specific oligonucleotide probes has recently been reported. The resulting DNA amplification was specific for the HLA-DPB locus. Typing for the individual DPB alleles was exclusively dependent on the hybridizations of the probes but hampered by close sequence homology between different DP alleles yielding complex patterns of reactivity with a panel of probes. We report the combined use of allele-specific DNA in vitro amplification and allele-specific oligonucleotides in typing for DPB1*03 and DPB1*06. Complete concordance with PLT typing was observed for the DPB1*03 alleles, while in the DPB1*06 group, at least three variant DPB1*06 alleles were identified which have not been described previously.
M3 - Journal article
C2 - 2777339
VL - 30
SP - 208
EP - 213
JO - Immunogenetics
JF - Immunogenetics
SN - 0093-7711
IS - 3
ER -
ID: 10637628