YeastFab: High-Throughput Genetic Parts Construction, Measurement, and Pathway Engineering in Yeast

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

YeastFab : High-Throughput Genetic Parts Construction, Measurement, and Pathway Engineering in Yeast. / Garcia-Ruiz, Eva; Auxillos, Jamie; Li, Tianyi; Dai, Junbiao; Cai, Yizhi.

Methods in Enzymology. ed. / Nigel Scrutton. Academic Press, 2018. p. 277-306 (Methods in Enzymology, Vol. 608).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Garcia-Ruiz, E, Auxillos, J, Li, T, Dai, J & Cai, Y 2018, YeastFab: High-Throughput Genetic Parts Construction, Measurement, and Pathway Engineering in Yeast. in N Scrutton (ed.), Methods in Enzymology. Academic Press, Methods in Enzymology, vol. 608, pp. 277-306. https://doi.org/10.1016/bs.mie.2018.05.003

APA

Garcia-Ruiz, E., Auxillos, J., Li, T., Dai, J., & Cai, Y. (2018). YeastFab: High-Throughput Genetic Parts Construction, Measurement, and Pathway Engineering in Yeast. In N. Scrutton (Ed.), Methods in Enzymology (pp. 277-306). Academic Press. Methods in Enzymology Vol. 608 https://doi.org/10.1016/bs.mie.2018.05.003

Vancouver

Garcia-Ruiz E, Auxillos J, Li T, Dai J, Cai Y. YeastFab: High-Throughput Genetic Parts Construction, Measurement, and Pathway Engineering in Yeast. In Scrutton N, editor, Methods in Enzymology. Academic Press. 2018. p. 277-306. (Methods in Enzymology, Vol. 608). https://doi.org/10.1016/bs.mie.2018.05.003

Author

Garcia-Ruiz, Eva ; Auxillos, Jamie ; Li, Tianyi ; Dai, Junbiao ; Cai, Yizhi. / YeastFab : High-Throughput Genetic Parts Construction, Measurement, and Pathway Engineering in Yeast. Methods in Enzymology. editor / Nigel Scrutton. Academic Press, 2018. pp. 277-306 (Methods in Enzymology, Vol. 608).

Bibtex

@inbook{a0098cbc55744d1584134752234bb0f6,
title = "YeastFab: High-Throughput Genetic Parts Construction, Measurement, and Pathway Engineering in Yeast",
abstract = "For many years, researchers have devised elegant techniques to assemble genetic parts into larger constructs. Recently, increasing needs for complex DNA constructs has driven countless attempts to optimize DNA assembly methods for improved efficiency, fidelity, and modularity. These efforts have resulted in simple, robust, standardized, and fast protocols that enable the implementation of high-throughput DNA assembly projects for the fabrication of large synthetic genetic constructs. Recently our groups have developed the YeastFab assembly, a highly efficient method for the design and construction of DNA-building blocks based on the native elements from Saccharomyces cerevisiae. Furthermore, these standardized DNA parts can be readily characterized and assembled into transcriptional units and pathways. In this chapter, we describe the protocols to assemble pathways from characterized standardized yeast parts using YeastFab.",
keywords = "DNA assembly, Metabolic engineering, Synthetic biology, Yeast, YeastFab",
author = "Eva Garcia-Ruiz and Jamie Auxillos and Tianyi Li and Junbiao Dai and Yizhi Cai",
note = "Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",
year = "2018",
month = jan,
day = "1",
doi = "10.1016/bs.mie.2018.05.003",
language = "English",
isbn = "9780128151488",
series = "Methods in Enzymology",
pages = "277--306",
editor = "Nigel Scrutton",
booktitle = "Methods in Enzymology",
publisher = "Academic Press",
address = "United States",

}

RIS

TY - CHAP

T1 - YeastFab

T2 - High-Throughput Genetic Parts Construction, Measurement, and Pathway Engineering in Yeast

AU - Garcia-Ruiz, Eva

AU - Auxillos, Jamie

AU - Li, Tianyi

AU - Dai, Junbiao

AU - Cai, Yizhi

N1 - Publisher Copyright: © 2018 Elsevier Inc.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - For many years, researchers have devised elegant techniques to assemble genetic parts into larger constructs. Recently, increasing needs for complex DNA constructs has driven countless attempts to optimize DNA assembly methods for improved efficiency, fidelity, and modularity. These efforts have resulted in simple, robust, standardized, and fast protocols that enable the implementation of high-throughput DNA assembly projects for the fabrication of large synthetic genetic constructs. Recently our groups have developed the YeastFab assembly, a highly efficient method for the design and construction of DNA-building blocks based on the native elements from Saccharomyces cerevisiae. Furthermore, these standardized DNA parts can be readily characterized and assembled into transcriptional units and pathways. In this chapter, we describe the protocols to assemble pathways from characterized standardized yeast parts using YeastFab.

AB - For many years, researchers have devised elegant techniques to assemble genetic parts into larger constructs. Recently, increasing needs for complex DNA constructs has driven countless attempts to optimize DNA assembly methods for improved efficiency, fidelity, and modularity. These efforts have resulted in simple, robust, standardized, and fast protocols that enable the implementation of high-throughput DNA assembly projects for the fabrication of large synthetic genetic constructs. Recently our groups have developed the YeastFab assembly, a highly efficient method for the design and construction of DNA-building blocks based on the native elements from Saccharomyces cerevisiae. Furthermore, these standardized DNA parts can be readily characterized and assembled into transcriptional units and pathways. In this chapter, we describe the protocols to assemble pathways from characterized standardized yeast parts using YeastFab.

KW - DNA assembly

KW - Metabolic engineering

KW - Synthetic biology

KW - Yeast

KW - YeastFab

UR - http://www.scopus.com/inward/record.url?scp=85049435989&partnerID=8YFLogxK

U2 - 10.1016/bs.mie.2018.05.003

DO - 10.1016/bs.mie.2018.05.003

M3 - Book chapter

C2 - 30173765

AN - SCOPUS:85049435989

SN - 9780128151488

T3 - Methods in Enzymology

SP - 277

EP - 306

BT - Methods in Enzymology

A2 - Scrutton, Nigel

PB - Academic Press

ER -

ID: 388827275