YeastFab: High-Throughput Genetic Parts Construction, Measurement, and Pathway Engineering in Yeast
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
For many years, researchers have devised elegant techniques to assemble genetic parts into larger constructs. Recently, increasing needs for complex DNA constructs has driven countless attempts to optimize DNA assembly methods for improved efficiency, fidelity, and modularity. These efforts have resulted in simple, robust, standardized, and fast protocols that enable the implementation of high-throughput DNA assembly projects for the fabrication of large synthetic genetic constructs. Recently our groups have developed the YeastFab assembly, a highly efficient method for the design and construction of DNA-building blocks based on the native elements from Saccharomyces cerevisiae. Furthermore, these standardized DNA parts can be readily characterized and assembled into transcriptional units and pathways. In this chapter, we describe the protocols to assemble pathways from characterized standardized yeast parts using YeastFab.
Original language | English |
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Title of host publication | Methods in Enzymology |
Editors | Nigel Scrutton |
Number of pages | 30 |
Publisher | Academic Press |
Publication date | 1 Jan 2018 |
Pages | 277-306 |
ISBN (Print) | 9780128151488 |
DOIs | |
Publication status | Published - 1 Jan 2018 |
Series | Methods in Enzymology |
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Volume | 608 |
ISSN | 0076-6879 |
Bibliographical note
Publisher Copyright:
© 2018 Elsevier Inc.
- DNA assembly, Metabolic engineering, Synthetic biology, Yeast, YeastFab
Research areas
ID: 388827275