Functional annotation of human long noncoding RNAs via molecular phenotyping
Research output: Contribution to journal › Journal article › Research › peer-review
Documents
- Functional annotation of human long noncoding RNAs via molecular phenotyping
Final published version, 8.69 MB, PDF document
Long noncoding RNAs (lncRNAs) constitute the majority of transcripts in the mammalian genomes, and yet, their functions remain largely unknown. As part of the FANTOM6 project, we systematically knocked down the expression of 285 lncRNAs in human dermal fibroblasts and quantified cellular growth, morphological changes, and transcriptomic responses using Capped Analysis of Gene Expression (CAGE). Antisense oligonucleotides targeting the same lncRNAs exhibited global concordance, and the molecular phenotype, measured by CAGE, recapitulated the observed cellular phenotypes while providing additional insights on the affected genes and pathways. Here, we disseminate the largest-todate lncRNA knockdown data set with molecular phenotyping (over 1000 CAGE deep-sequencing libraries) for further exploration and highlight functional roles for ZNF213-AS1 and lnc-KHDC3L-2.
Original language | English |
---|---|
Journal | Genome Research |
Volume | 30 |
Issue number | 7 |
Pages (from-to) | 1060-1072 |
Number of pages | 13 |
ISSN | 1088-9051 |
DOIs | |
Publication status | Published - 2020 |
- ADULT HUMAN FIBROBLASTS, GENE-EXPRESSION, UNIQUE FEATURES, CELLS, TRANSCRIPTION, REVEALS, DIFFERENTIATION, TRANSLATION, PRINCIPLES, REGULATORS
Research areas
Number of downloads are based on statistics from Google Scholar and www.ku.dk
ID: 247158244