Cell transcriptomic atlas of the non-human primate Macaca fascicularis

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  • Lei Han
  • Xiaoyu Wei
  • Chuanyu Liu
  • Giacomo Volpe
  • Zhenkun Zhuang
  • Xuanxuan Zou
  • Zhifeng Wang
  • Taotao Pan
  • Yue Yuan
  • Xiao Zhang
  • Peng Fan
  • Pengcheng Guo
  • Yiwei Lai
  • Ying Lei
  • Xingyuan Liu
  • Feng Yu
  • Shuncheng Shangguan
  • Guangyao Lai
  • Qiuting Deng
  • Ya Liu
  • Liang Wu
  • Hao Yu
  • Yunting Huang
  • Mengnan Cheng
  • Jiangshan Xu
  • Yang Liu
  • Mingyue Wang
  • Chunqing Wang
  • Yuanhang Zhang
  • Duo Xie
  • Yunzhi Yang
  • Yeya Yu
  • Huiwen Zheng
  • Yanrong Wei
  • Fubaoqian Huang
  • Junjie Lei
  • Waidong Huang
  • Zhiyong Zhu
  • Haorong Lu
  • Bo Wang
  • Xiaofeng Wei
  • Fengzhen Chen
  • Tao Yang
  • Wensi Du
  • Jing Chen
  • Shibo Xu
  • Juan An
  • Carl Ward
  • Zongren Wang
  • Zhong Pei
  • Chi-Wai Wong
  • Xiaolei Liu
  • Huafeng Zhang
  • Mingyuan Liu
  • Baoming Qin
  • Axel Schambach
  • Joan Isern
  • Liqiang Feng
  • Yan Liu
  • Xiangyu Guo
  • Zhen Liu
  • Qiang Sun
  • Patrick H. Maxwell
  • Nick Barker
  • Pura Muñoz-Cánoves
  • Ying Gu
  • Jan Mulder
  • Mathias Uhlen
  • Tao Tan
  • Shiping Liu
  • Huanming Yang
  • Jian Wang
  • Yong Hou
  • Xun Xu
  • Miguel A. Esteban
  • Longqi Liu

Studying tissue composition and function in non-human primates (NHPs) is crucial to understand the nature of our own species. Here we present a large-scale cell transcriptomic atlas that encompasses over 1 million cells from 45 tissues of the adult NHP Macaca fascicularis. This dataset provides a vast annotated resource to study a species phylogenetically close to humans. To demonstrate the utility of the atlas, we have reconstructed the cell–cell interaction networks that drive Wnt signalling across the body, mapped the distribution of receptors and co-receptors for viruses causing human infectious diseases, and intersected our data with human genetic disease orthologues to establish potential clinical associations. Our M. fascicularis cell atlas constitutes an essential reference for future studies in humans and NHPs.

OriginalsprogEngelsk
TidsskriftNature
Vol/bind604
Sider (fra-til)723-731
ISSN0028-0836
DOI
StatusUdgivet - 2022

Bibliografisk note

Funding Information:
We thank W. Liu and L. Xu from the Huazhen Laboratory Animal Breeding Centre for helping in the collection of monkey tissues, D. Zhu and H. Li from the Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory) for technical help, G. Guo and H. Sun from Zhejiang University for providing HCL and MCA gene expression data matrices, G. Dong and C. Liu from BGI Research, and X. Zhang, P. Li and C. Qi from the Guangzhou Institutes of Biomedicine and Health for experimental advice or providing reagents. This work was supported by the Shenzhen Basic Research Project for Excellent Young Scholars (RCYX20200714114644191), Shenzhen Key Laboratory of Single-Cell Omics (ZDSYS20190902093613831), Shenzhen Bay Laboratory (SZBL2019062801012) and Guangdong Provincial Key Laboratory of Genome Read and Write (2017B030301011). In addition, L.L. was supported by the National Natural Science Foundation of China (31900466), Y. Hou was supported by the Natural Science Foundation of Guangdong Province (2018A030313379) and M.A.E. was supported by a Changbai Mountain Scholar award (419020201252), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16030502), a Chinese Academy of Sciences–Japan Society for the Promotion of Science joint research project (GJHZ2093), the National Natural Science Foundation of China (92068106, U20A2015) and the Guangdong Basic and Applied Basic Research Foundation (2021B1515120075). M.L. was supported by the National Key Research and Development Program of China (2021YFC2600200).

Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature Limited.

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