The airway microbiota of neonates colonized with asthma-associated pathogenic bacteria

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The airway microbiota of neonates colonized with asthma-associated pathogenic bacteria. / Thorsen, Jonathan; Li, Xuan Ji; Peng, Shuang; Sunde, Rikke Bjersand; Shah, Shiraz A.; Bhattacharyya, Madhumita; Poulsen, Casper Sahl; Poulsen, Christina Egeø; Leal Rodriguez, Cristina; Widdowson, Michael; Neumann, Avidan Uriel; Trivedi, Urvish; Chawes, Bo; Bønnelykke, Klaus; Bisgaard, Hans; Sørensen, Søren J.; Stokholm, Jakob.

In: Nature Communications, Vol. 14, 6668, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Thorsen, J, Li, XJ, Peng, S, Sunde, RB, Shah, SA, Bhattacharyya, M, Poulsen, CS, Poulsen, CE, Leal Rodriguez, C, Widdowson, M, Neumann, AU, Trivedi, U, Chawes, B, Bønnelykke, K, Bisgaard, H, Sørensen, SJ & Stokholm, J 2023, 'The airway microbiota of neonates colonized with asthma-associated pathogenic bacteria', Nature Communications, vol. 14, 6668. https://doi.org/10.1038/s41467-023-42309-z

APA

Thorsen, J., Li, X. J., Peng, S., Sunde, R. B., Shah, S. A., Bhattacharyya, M., Poulsen, C. S., Poulsen, C. E., Leal Rodriguez, C., Widdowson, M., Neumann, A. U., Trivedi, U., Chawes, B., Bønnelykke, K., Bisgaard, H., Sørensen, S. J., & Stokholm, J. (2023). The airway microbiota of neonates colonized with asthma-associated pathogenic bacteria. Nature Communications, 14, [6668]. https://doi.org/10.1038/s41467-023-42309-z

Vancouver

Thorsen J, Li XJ, Peng S, Sunde RB, Shah SA, Bhattacharyya M et al. The airway microbiota of neonates colonized with asthma-associated pathogenic bacteria. Nature Communications. 2023;14. 6668. https://doi.org/10.1038/s41467-023-42309-z

Author

Thorsen, Jonathan ; Li, Xuan Ji ; Peng, Shuang ; Sunde, Rikke Bjersand ; Shah, Shiraz A. ; Bhattacharyya, Madhumita ; Poulsen, Casper Sahl ; Poulsen, Christina Egeø ; Leal Rodriguez, Cristina ; Widdowson, Michael ; Neumann, Avidan Uriel ; Trivedi, Urvish ; Chawes, Bo ; Bønnelykke, Klaus ; Bisgaard, Hans ; Sørensen, Søren J. ; Stokholm, Jakob. / The airway microbiota of neonates colonized with asthma-associated pathogenic bacteria. In: Nature Communications. 2023 ; Vol. 14.

Bibtex

@article{e7ab9b38551143fcb6c1c3e15ef6f5ca,

title = "The airway microbiota of neonates colonized with asthma-associated pathogenic bacteria",

abstract = "Culture techniques have associated colonization with pathogenic bacteria in the airways of neonates with later risk of childhood asthma, whereas more recent studies utilizing sequencing techniques have shown the same phenomenon with specific anaerobic taxa. Here, we analyze nasopharyngeal swabs from 1 month neonates in the COPSAC2000 prospective birth cohort by 16S rRNA gene sequencing of the V3-V4 region in relation to asthma risk throughout childhood. Results are compared with previous culture results from hypopharyngeal aspirates from the same cohort and with hypopharyngeal sequencing data from the later COPSAC2010 cohort. Nasopharyngeal relative abundance values of Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis are associated with the same species in the hypopharyngeal cultures. A combined pathogen score of these bacteria{\textquoteright}s abundance values is associated with persistent wheeze/asthma by age 7. No other taxa are associated. Compared to the hypopharyngeal aspirates from the COPSAC2010 cohort, the anaerobes Veillonella and Prevotella, which have previously been implicated in asthma development, are less commonly detected in the COPSAC2000 nasopharyngeal samples, but correlate with the pathogen score, hinting at latent community structures that bridge current and previous results. These findings have implications for future asthma prevention efforts.",

author = "Jonathan Thorsen and Li, {Xuan Ji} and Shuang Peng and Sunde, {Rikke Bjersand} and Shah, {Shiraz A.} and Madhumita Bhattacharyya and Poulsen, {Casper Sahl} and Poulsen, {Christina Ege{\o}} and {Leal Rodriguez}, Cristina and Michael Widdowson and Neumann, {Avidan Uriel} and Urvish Trivedi and Bo Chawes and Klaus B{\o}nnelykke and Hans Bisgaard and S{\o}rensen, {S{\o}ren J.} and Jakob Stokholm",

note = "Publisher Copyright: {\textcopyright} 2023, Springer Nature Limited.",

year = "2023",

doi = "10.1038/s41467-023-42309-z",

language = "English",

volume = "14",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - The airway microbiota of neonates colonized with asthma-associated pathogenic bacteria

AU - Thorsen, Jonathan

AU - Li, Xuan Ji

AU - Peng, Shuang

AU - Sunde, Rikke Bjersand

AU - Shah, Shiraz A.

AU - Bhattacharyya, Madhumita

AU - Poulsen, Casper Sahl

AU - Poulsen, Christina Egeø

AU - Leal Rodriguez, Cristina

AU - Widdowson, Michael

AU - Neumann, Avidan Uriel

AU - Trivedi, Urvish

AU - Chawes, Bo

AU - Bønnelykke, Klaus

AU - Bisgaard, Hans

AU - Sørensen, Søren J.

AU - Stokholm, Jakob

PY - 2023

Y1 - 2023

N2 - Culture techniques have associated colonization with pathogenic bacteria in the airways of neonates with later risk of childhood asthma, whereas more recent studies utilizing sequencing techniques have shown the same phenomenon with specific anaerobic taxa. Here, we analyze nasopharyngeal swabs from 1 month neonates in the COPSAC2000 prospective birth cohort by 16S rRNA gene sequencing of the V3-V4 region in relation to asthma risk throughout childhood. Results are compared with previous culture results from hypopharyngeal aspirates from the same cohort and with hypopharyngeal sequencing data from the later COPSAC2010 cohort. Nasopharyngeal relative abundance values of Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis are associated with the same species in the hypopharyngeal cultures. A combined pathogen score of these bacteria’s abundance values is associated with persistent wheeze/asthma by age 7. No other taxa are associated. Compared to the hypopharyngeal aspirates from the COPSAC2010 cohort, the anaerobes Veillonella and Prevotella, which have previously been implicated in asthma development, are less commonly detected in the COPSAC2000 nasopharyngeal samples, but correlate with the pathogen score, hinting at latent community structures that bridge current and previous results. These findings have implications for future asthma prevention efforts.

AB - Culture techniques have associated colonization with pathogenic bacteria in the airways of neonates with later risk of childhood asthma, whereas more recent studies utilizing sequencing techniques have shown the same phenomenon with specific anaerobic taxa. Here, we analyze nasopharyngeal swabs from 1 month neonates in the COPSAC2000 prospective birth cohort by 16S rRNA gene sequencing of the V3-V4 region in relation to asthma risk throughout childhood. Results are compared with previous culture results from hypopharyngeal aspirates from the same cohort and with hypopharyngeal sequencing data from the later COPSAC2010 cohort. Nasopharyngeal relative abundance values of Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis are associated with the same species in the hypopharyngeal cultures. A combined pathogen score of these bacteria’s abundance values is associated with persistent wheeze/asthma by age 7. No other taxa are associated. Compared to the hypopharyngeal aspirates from the COPSAC2010 cohort, the anaerobes Veillonella and Prevotella, which have previously been implicated in asthma development, are less commonly detected in the COPSAC2000 nasopharyngeal samples, but correlate with the pathogen score, hinting at latent community structures that bridge current and previous results. These findings have implications for future asthma prevention efforts.

U2 - 10.1038/s41467-023-42309-z

DO - 10.1038/s41467-023-42309-z

M3 - Journal article

C2 - 37863895

AN - SCOPUS:85174635805

VL - 14

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 6668

ER -

ID: 371923051

Department of Biology