A Sleeping Beauty DNA transposon-based genetic sensor for functional screening of vitamin D3 analogues

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

A Sleeping Beauty DNA transposon-based genetic sensor for functional screening of vitamin D3 analogues. / Staunstrup, Nicklas Heine; Sharma, Nynne; Bak, Rasmus Otkjær; Svensson, Lars; Petersen, Thomas K. ; Aarenstrup, Lene; Kristiansen, Karsten; Bolund, Lars; Mikkelsen, Jacob Giehm.

In: B M C Biotechnology, Vol. 11, 2011.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Staunstrup, NH, Sharma, N, Bak, RO, Svensson, L, Petersen, TK, Aarenstrup, L, Kristiansen, K, Bolund, L & Mikkelsen, JG 2011, 'A Sleeping Beauty DNA transposon-based genetic sensor for functional screening of vitamin D3 analogues', B M C Biotechnology, vol. 11. https://doi.org/10.1186/1472-6750-11-33

APA

Staunstrup, N. H., Sharma, N., Bak, R. O., Svensson, L., Petersen, T. K., Aarenstrup, L., Kristiansen, K., Bolund, L., & Mikkelsen, J. G. (2011). A Sleeping Beauty DNA transposon-based genetic sensor for functional screening of vitamin D3 analogues. B M C Biotechnology, 11. https://doi.org/10.1186/1472-6750-11-33

Vancouver

Staunstrup NH, Sharma N, Bak RO, Svensson L, Petersen TK, Aarenstrup L et al. A Sleeping Beauty DNA transposon-based genetic sensor for functional screening of vitamin D3 analogues. B M C Biotechnology. 2011;11. https://doi.org/10.1186/1472-6750-11-33

Author

Staunstrup, Nicklas Heine ; Sharma, Nynne ; Bak, Rasmus Otkjær ; Svensson, Lars ; Petersen, Thomas K. ; Aarenstrup, Lene ; Kristiansen, Karsten ; Bolund, Lars ; Mikkelsen, Jacob Giehm. / A Sleeping Beauty DNA transposon-based genetic sensor for functional screening of vitamin D3 analogues. In: B M C Biotechnology. 2011 ; Vol. 11.

Bibtex

@article{b1d2912734634f28acfb11b0455e86ec,
title = "A Sleeping Beauty DNA transposon-based genetic sensor for functional screening of vitamin D3 analogues",
abstract = "Analogues of vitamin D3 are extensively used in the treatment of various illnesses, such as osteoporosis, inflammatory skin diseases, and cancer. Functional testing of new vitamin D3 analogues and formulations for improved systemic and topical administration is supported by sensitive screening methods that allow a comparative evaluation of drug properties. As a new tool in functional screening of vitamin D3 analogues, we describe a genomically integratable sensor for sensitive drug detection. This system facilitates assessment of the pharmacokinetic and pharmadynamic properties of vitamin D3 analogues. The tri-cistronic genetic sensor encodes a drug-sensoring protein, a reporter protein expressed from an activated sensor-responsive promoter, and a resistance marker.",
keywords = "Animals, Calcitriol, Cell Line, Cell Proliferation, Cell Survival, Cholecalciferol, DNA Transposable Elements, Dose-Response Relationship, Drug, Drug Evaluation, Preclinical, Genetic Vectors, Green Fluorescent Proteins, Humans, Hydroxycholecalciferols, Keratinocytes, Microscopy, Fluorescence, Mutagenesis, Insertional, Response Elements, Retinoid X Receptors, Transfection, Transposases, Vitamin D-Binding Protein",
author = "Staunstrup, {Nicklas Heine} and Nynne Sharma and Bak, {Rasmus Otkj{\ae}r} and Lars Svensson and Petersen, {Thomas K.} and Lene Aarenstrup and Karsten Kristiansen and Lars Bolund and Mikkelsen, {Jacob Giehm}",
year = "2011",
doi = "10.1186/1472-6750-11-33",
language = "English",
volume = "11",
journal = "BMC Biotechnology",
issn = "1472-6750",
publisher = "BioMed Central Ltd.",

}

RIS

TY - JOUR

T1 - A Sleeping Beauty DNA transposon-based genetic sensor for functional screening of vitamin D3 analogues

AU - Staunstrup, Nicklas Heine

AU - Sharma, Nynne

AU - Bak, Rasmus Otkjær

AU - Svensson, Lars

AU - Petersen, Thomas K.

AU - Aarenstrup, Lene

AU - Kristiansen, Karsten

AU - Bolund, Lars

AU - Mikkelsen, Jacob Giehm

PY - 2011

Y1 - 2011

N2 - Analogues of vitamin D3 are extensively used in the treatment of various illnesses, such as osteoporosis, inflammatory skin diseases, and cancer. Functional testing of new vitamin D3 analogues and formulations for improved systemic and topical administration is supported by sensitive screening methods that allow a comparative evaluation of drug properties. As a new tool in functional screening of vitamin D3 analogues, we describe a genomically integratable sensor for sensitive drug detection. This system facilitates assessment of the pharmacokinetic and pharmadynamic properties of vitamin D3 analogues. The tri-cistronic genetic sensor encodes a drug-sensoring protein, a reporter protein expressed from an activated sensor-responsive promoter, and a resistance marker.

AB - Analogues of vitamin D3 are extensively used in the treatment of various illnesses, such as osteoporosis, inflammatory skin diseases, and cancer. Functional testing of new vitamin D3 analogues and formulations for improved systemic and topical administration is supported by sensitive screening methods that allow a comparative evaluation of drug properties. As a new tool in functional screening of vitamin D3 analogues, we describe a genomically integratable sensor for sensitive drug detection. This system facilitates assessment of the pharmacokinetic and pharmadynamic properties of vitamin D3 analogues. The tri-cistronic genetic sensor encodes a drug-sensoring protein, a reporter protein expressed from an activated sensor-responsive promoter, and a resistance marker.

KW - Animals

KW - Calcitriol

KW - Cell Line

KW - Cell Proliferation

KW - Cell Survival

KW - Cholecalciferol

KW - DNA Transposable Elements

KW - Dose-Response Relationship, Drug

KW - Drug Evaluation, Preclinical

KW - Genetic Vectors

KW - Green Fluorescent Proteins

KW - Humans

KW - Hydroxycholecalciferols

KW - Keratinocytes

KW - Microscopy, Fluorescence

KW - Mutagenesis, Insertional

KW - Response Elements

KW - Retinoid X Receptors

KW - Transfection

KW - Transposases

KW - Vitamin D-Binding Protein

U2 - 10.1186/1472-6750-11-33

DO - 10.1186/1472-6750-11-33

M3 - Journal article

C2 - 21473770

VL - 11

JO - BMC Biotechnology

JF - BMC Biotechnology

SN - 1472-6750

ER -

ID: 35410253