Inherent lack of CMFDA/FDA staining in certain algae and its implication for ballast water testing

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Inherent lack of CMFDA/FDA staining in certain algae and its implication for ballast water testing. / Hansen, Gert; Petersen, Gitte; Maguet, Rémi; Sim, Esther; Madsen, Torben.

In: Marine Pollution Bulletin, Vol. 194, No. Part B, 115312, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hansen, G, Petersen, G, Maguet, R, Sim, E & Madsen, T 2023, 'Inherent lack of CMFDA/FDA staining in certain algae and its implication for ballast water testing', Marine Pollution Bulletin, vol. 194, no. Part B, 115312. https://doi.org/10.1016/j.marpolbul.2023.115312

APA

Hansen, G., Petersen, G., Maguet, R., Sim, E., & Madsen, T. (2023). Inherent lack of CMFDA/FDA staining in certain algae and its implication for ballast water testing. Marine Pollution Bulletin, 194(Part B), [115312]. https://doi.org/10.1016/j.marpolbul.2023.115312

Vancouver

Hansen G, Petersen G, Maguet R, Sim E, Madsen T. Inherent lack of CMFDA/FDA staining in certain algae and its implication for ballast water testing. Marine Pollution Bulletin. 2023;194(Part B). 115312. https://doi.org/10.1016/j.marpolbul.2023.115312

Author

Hansen, Gert ; Petersen, Gitte ; Maguet, Rémi ; Sim, Esther ; Madsen, Torben. / Inherent lack of CMFDA/FDA staining in certain algae and its implication for ballast water testing. In: Marine Pollution Bulletin. 2023 ; Vol. 194, No. Part B.

Bibtex

@article{5f213e3cd95a4df2b8edbaedeecd3a6a,

title = "Inherent lack of CMFDA/FDA staining in certain algae and its implication for ballast water testing",

abstract = "The approved method for testing the efficacy of ballast water management systems with respect to killing 10-50 μm organisms uses movements of the organisms or the vital stains CMFDA/FDA. The present study demonstrates that certain freshwater coccoid chlorophytes, known or suspected to contain a highly resistant cell wall component (algaenan), stain poorly with CMFDA/FDA, resulting in false negatives. The staining rates for the most dominant species were determined and were approx. 3-70 %. The use of Crystal Violet as an indicator for the presence of algaenan gave inconclusive results. The number of the 10-50 μm organisms in a small pond was found to be 10,183 organisms/mL (Lugol's fixed sample) vs. 2335 organisms/mL (CMFDA/FDA-stained sample). Using the staining rates obtained, it was estimated that the number of false negatives could make 40-50 %. The implications for biological performance evaluation of ballast water management systems are discussed.",

author = "Gert Hansen and Gitte Petersen and R{\'e}mi Maguet and Esther Sim and Torben Madsen",

year = "2023",

doi = "10.1016/j.marpolbul.2023.115312",

language = "English",

volume = "194",

journal = "Marine Pollution Bulletin",

issn = "0025-326X",

publisher = "Elsevier",

number = "Part B",

}

RIS

TY - JOUR

T1 - Inherent lack of CMFDA/FDA staining in certain algae and its implication for ballast water testing

AU - Hansen, Gert

AU - Petersen, Gitte

AU - Maguet, Rémi

AU - Sim, Esther

AU - Madsen, Torben

PY - 2023

Y1 - 2023

N2 - The approved method for testing the efficacy of ballast water management systems with respect to killing 10-50 μm organisms uses movements of the organisms or the vital stains CMFDA/FDA. The present study demonstrates that certain freshwater coccoid chlorophytes, known or suspected to contain a highly resistant cell wall component (algaenan), stain poorly with CMFDA/FDA, resulting in false negatives. The staining rates for the most dominant species were determined and were approx. 3-70 %. The use of Crystal Violet as an indicator for the presence of algaenan gave inconclusive results. The number of the 10-50 μm organisms in a small pond was found to be 10,183 organisms/mL (Lugol's fixed sample) vs. 2335 organisms/mL (CMFDA/FDA-stained sample). Using the staining rates obtained, it was estimated that the number of false negatives could make 40-50 %. The implications for biological performance evaluation of ballast water management systems are discussed.

AB - The approved method for testing the efficacy of ballast water management systems with respect to killing 10-50 μm organisms uses movements of the organisms or the vital stains CMFDA/FDA. The present study demonstrates that certain freshwater coccoid chlorophytes, known or suspected to contain a highly resistant cell wall component (algaenan), stain poorly with CMFDA/FDA, resulting in false negatives. The staining rates for the most dominant species were determined and were approx. 3-70 %. The use of Crystal Violet as an indicator for the presence of algaenan gave inconclusive results. The number of the 10-50 μm organisms in a small pond was found to be 10,183 organisms/mL (Lugol's fixed sample) vs. 2335 organisms/mL (CMFDA/FDA-stained sample). Using the staining rates obtained, it was estimated that the number of false negatives could make 40-50 %. The implications for biological performance evaluation of ballast water management systems are discussed.

U2 - 10.1016/j.marpolbul.2023.115312

DO - 10.1016/j.marpolbul.2023.115312

M3 - Journal article

C2 - 37481801

VL - 194

JO - Marine Pollution Bulletin

JF - Marine Pollution Bulletin

SN - 0025-326X

IS - Part B

M1 - 115312

ER -

ID: 360135181

Department of Biology