Inherent lack of CMFDA/FDA staining in certain algae and its implication for ballast water testing
Research output: Contribution to journal › Journal article › Research › peer-review
The approved method for testing the efficacy of ballast water management systems with respect to killing 10-50 μm organisms uses movements of the organisms or the vital stains CMFDA/FDA. The present study demonstrates that certain freshwater coccoid chlorophytes, known or suspected to contain a highly resistant cell wall component (algaenan), stain poorly with CMFDA/FDA, resulting in false negatives. The staining rates for the most dominant species were determined and were approx. 3-70 %. The use of Crystal Violet as an indicator for the presence of algaenan gave inconclusive results. The number of the 10-50 μm organisms in a small pond was found to be 10,183 organisms/mL (Lugol's fixed sample) vs. 2335 organisms/mL (CMFDA/FDA-stained sample). Using the staining rates obtained, it was estimated that the number of false negatives could make 40-50 %. The implications for biological performance evaluation of ballast water management systems are discussed.
Original language | English |
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Article number | 115312 |
Journal | Marine Pollution Bulletin |
Volume | 194 |
Issue number | Part B |
Number of pages | 9 |
ISSN | 0025-326X |
DOIs | |
Publication status | Published - 2023 |
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ID: 360135181