Ultrastructural localization of RFamide-like peptides in neuronal dense-cored vesicles in the peduncle of Hydra

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Ultrastructural localization of RFamide-like peptides in neuronal dense-cored vesicles in the peduncle of Hydra. / Koizumi, O; Wilson, Douglas Clyde; Grimmelikhuijzen, C J; Westfall, J A.

In: The Journal of experimental zoology, Vol. 249, No. 1, 01.01.1989, p. 17-22.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Koizumi, O, Wilson, DC, Grimmelikhuijzen, CJ & Westfall, JA 1989, 'Ultrastructural localization of RFamide-like peptides in neuronal dense-cored vesicles in the peduncle of Hydra', The Journal of experimental zoology, vol. 249, no. 1, pp. 17-22. https://doi.org/10.1002/jez.1402490105

APA

Koizumi, O., Wilson, D. C., Grimmelikhuijzen, C. J., & Westfall, J. A. (1989). Ultrastructural localization of RFamide-like peptides in neuronal dense-cored vesicles in the peduncle of Hydra. The Journal of experimental zoology, 249(1), 17-22. https://doi.org/10.1002/jez.1402490105

Vancouver

Koizumi O, Wilson DC, Grimmelikhuijzen CJ, Westfall JA. Ultrastructural localization of RFamide-like peptides in neuronal dense-cored vesicles in the peduncle of Hydra. The Journal of experimental zoology. 1989 Jan 1;249(1):17-22. https://doi.org/10.1002/jez.1402490105

Author

Koizumi, O ; Wilson, Douglas Clyde ; Grimmelikhuijzen, C J ; Westfall, J A. / Ultrastructural localization of RFamide-like peptides in neuronal dense-cored vesicles in the peduncle of Hydra. In: The Journal of experimental zoology. 1989 ; Vol. 249, No. 1. pp. 17-22.

Bibtex

@article{972dbc2d3f43408387c1dc19c84ea3ee,
title = "Ultrastructural localization of RFamide-like peptides in neuronal dense-cored vesicles in the peduncle of Hydra",
abstract = "The presence of Arg-Phe-amide (RFamide)-like peptides in dense-cored vesicles in neurons of the peduncle of Hydra was demonstrated by immunogold electron microscopy. Thin sections of Lowicryl-embedded tissue labeled with antisera to RFamide and 5-nm gold-conjugated, secondary antibody and of Epon-Araldite-embedded tissue labeled with 15-nm gold particles revealed a concentration of RFamide-like immunoreactivity over the granular cores of vesicles in epidermal ganglion cells. Gold-labeled, dense-cored vesicles were present in the perikaryon, long thin neurites, and axon terminals of these neurons. The aggregation of labeled dense-cored vesicles in an axon terminal on the myoneme of an epitheliomuscular cell suggests a possible function of RFamide-like peptides in neuromuscular transmission. Gold staining of dense-cored vesicles completely disappeared when the RFamide antiserum was preabsorbed with 10 micrograms/ml RFamide. These results are the first demonstration that the dense-cored vesicles of Hydra neurons contain a neuropeptide.",
keywords = "Animals, Hydra, Immunohistochemistry, Microscopy, Electron, Neuropeptides",
author = "O Koizumi and Wilson, {Douglas Clyde} and Grimmelikhuijzen, {C J} and Westfall, {J A}",
year = "1989",
month = jan,
day = "1",
doi = "10.1002/jez.1402490105",
language = "English",
volume = "249",
pages = "17--22",
journal = "Journal of Experimental Zoology",
issn = "0022-104X",
publisher = "Wiley-Liss Inc.",
number = "1",

}

RIS

TY - JOUR

T1 - Ultrastructural localization of RFamide-like peptides in neuronal dense-cored vesicles in the peduncle of Hydra

AU - Koizumi, O

AU - Wilson, Douglas Clyde

AU - Grimmelikhuijzen, C J

AU - Westfall, J A

PY - 1989/1/1

Y1 - 1989/1/1

N2 - The presence of Arg-Phe-amide (RFamide)-like peptides in dense-cored vesicles in neurons of the peduncle of Hydra was demonstrated by immunogold electron microscopy. Thin sections of Lowicryl-embedded tissue labeled with antisera to RFamide and 5-nm gold-conjugated, secondary antibody and of Epon-Araldite-embedded tissue labeled with 15-nm gold particles revealed a concentration of RFamide-like immunoreactivity over the granular cores of vesicles in epidermal ganglion cells. Gold-labeled, dense-cored vesicles were present in the perikaryon, long thin neurites, and axon terminals of these neurons. The aggregation of labeled dense-cored vesicles in an axon terminal on the myoneme of an epitheliomuscular cell suggests a possible function of RFamide-like peptides in neuromuscular transmission. Gold staining of dense-cored vesicles completely disappeared when the RFamide antiserum was preabsorbed with 10 micrograms/ml RFamide. These results are the first demonstration that the dense-cored vesicles of Hydra neurons contain a neuropeptide.

AB - The presence of Arg-Phe-amide (RFamide)-like peptides in dense-cored vesicles in neurons of the peduncle of Hydra was demonstrated by immunogold electron microscopy. Thin sections of Lowicryl-embedded tissue labeled with antisera to RFamide and 5-nm gold-conjugated, secondary antibody and of Epon-Araldite-embedded tissue labeled with 15-nm gold particles revealed a concentration of RFamide-like immunoreactivity over the granular cores of vesicles in epidermal ganglion cells. Gold-labeled, dense-cored vesicles were present in the perikaryon, long thin neurites, and axon terminals of these neurons. The aggregation of labeled dense-cored vesicles in an axon terminal on the myoneme of an epitheliomuscular cell suggests a possible function of RFamide-like peptides in neuromuscular transmission. Gold staining of dense-cored vesicles completely disappeared when the RFamide antiserum was preabsorbed with 10 micrograms/ml RFamide. These results are the first demonstration that the dense-cored vesicles of Hydra neurons contain a neuropeptide.

KW - Animals

KW - Hydra

KW - Immunohistochemistry

KW - Microscopy, Electron

KW - Neuropeptides

U2 - 10.1002/jez.1402490105

DO - 10.1002/jez.1402490105

M3 - Journal article

C2 - 2926357

VL - 249

SP - 17

EP - 22

JO - Journal of Experimental Zoology

JF - Journal of Experimental Zoology

SN - 0022-104X

IS - 1

ER -

ID: 33514299