Chromosomal Fragile Sites and Extremely Large Genes – Oestergaard group

Common chromosomal fragile sites (CFSs) are regions of the genome that are highly unstable. CFSs host extremely large genes. We are trying to understand why extremely large genes cause genomic instability and how mutations arise at CFSs. Moreover, we aim to unravel the biological function of CFSs.

We use chicken and human cell lines as model systems. Our approach is mainly based on molecular genetics/CRISPR-Cas9, which we combine with a wide range of techniques such as fluorescence microscopy of living or immunostained cells, FISH, clonogenic survival, western blot, molecular cloning and qPCR.