Molecular identification of a myosuppressin receptor from the malaria mosquito Anopheles gambiae.
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The insect myosuppressins (X1DVX2HX3FLRFamide) are neuropeptides that generally block insect muscle activities. We have used the genomic sequence information from the malaria mosquito Anopheles gambiae Genome Project to clone a G protein-coupled receptor that was closely related to the two previously cloned and characterized myosuppressin receptors from Drosophila [Proc. Natl. Acad. Sci. USA 100 (2003) 9808]. The mosquito receptor cDNA was expressed in Chinese hamster ovary cells and was found to be activated by low concentrations of Anopheles myosuppressin (TDVDHVFLRFamide; EC50, 1.6 x 10(-8)M). The receptor was not activated by a library of 35 other insect neuropeptides and monoamines, including neuropeptides that resembled myosuppressin in their C-terminal moiety, such as PDRNFLRFamide (Anopheles FMRFamide-3), other Anopheles FMRFamide peptides, or neuropeptide F-like peptides, showing that the receptor was quite selective for myosuppressin. These results also showed that the myosuppressin receptor needs a much larger portion than the C-terminal FLRFamide sequence for its activation. The insect myosuppressins are often grouped together with the insect FMRFamides under the name FaRPs (FMRFamide-related peptides). However, this is not justified anymore, because the insect myosuppressin receptor/ligand couple is both functionally and evolutionarily fully unrelated to the insect FMRFamide receptor/ligand couple. To our knowledge, this is the first report on the molecular identification of a mosquito neuropeptide receptor.
Original language | English |
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Journal | Biochemical and Biophysical Research Communications |
Volume | 327 |
Issue number | 1 |
Pages (from-to) | 29-34 |
Number of pages | 5 |
ISSN | 0006-291X |
DOIs | |
Publication status | Published - 2005 |
Bibliographical note
Keywords: Amino Acid Sequence; Animals; Anopheles gambiae; Base Sequence; CHO Cells; Cloning, Molecular; Cricetinae; DNA, Complementary; Exons; Introns; Molecular Sequence Data; Receptors, Neuropeptide; Sequence Alignment
ID: 3045835