Archaeal viruses of the sulfolobales: isolation, infection, and CRISPR spacer acquisition

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

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Archaeal viruses of the sulfolobales : isolation, infection, and CRISPR spacer acquisition. / Erdmann, Susanne; Garrett, Roger Antony.

CRISPR: methods and protocols. ed. / Magnus Lundgren; Emmanuelle Charpentier; Peter C. Fineran. Springer, 2015. p. 223-232 (Methods in Molecular Biology, Vol. 1311).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Erdmann, S & Garrett, RA 2015, Archaeal viruses of the sulfolobales: isolation, infection, and CRISPR spacer acquisition. in M Lundgren, E Charpentier & PC Fineran (eds), CRISPR: methods and protocols. Springer, Methods in Molecular Biology, vol. 1311, pp. 223-232. https://doi.org/10.1007/978-1-4939-2687-9_14

APA

Erdmann, S., & Garrett, R. A. (2015). Archaeal viruses of the sulfolobales: isolation, infection, and CRISPR spacer acquisition. In M. Lundgren, E. Charpentier, & P. C. Fineran (Eds.), CRISPR: methods and protocols (pp. 223-232). Springer. Methods in Molecular Biology Vol. 1311 https://doi.org/10.1007/978-1-4939-2687-9_14

Vancouver

Erdmann S, Garrett RA. Archaeal viruses of the sulfolobales: isolation, infection, and CRISPR spacer acquisition. In Lundgren M, Charpentier E, Fineran PC, editors, CRISPR: methods and protocols. Springer. 2015. p. 223-232. (Methods in Molecular Biology, Vol. 1311). https://doi.org/10.1007/978-1-4939-2687-9_14

Author

Erdmann, Susanne ; Garrett, Roger Antony. / Archaeal viruses of the sulfolobales : isolation, infection, and CRISPR spacer acquisition. CRISPR: methods and protocols. editor / Magnus Lundgren ; Emmanuelle Charpentier ; Peter C. Fineran. Springer, 2015. pp. 223-232 (Methods in Molecular Biology, Vol. 1311).

Bibtex

@inbook{b543708f8d914f88b57b3bb40e94c952,
title = "Archaeal viruses of the sulfolobales: isolation, infection, and CRISPR spacer acquisition",
abstract = "Infection of archaea with phylogenetically diverse single viruses, performed in different laboratories, has failed to activate spacer acquisition into host CRISPR loci. The first successful uptake of archaeal de novo spacers was observed on infection of Sulfolobus solfataricus P2 with an environmental virus mixture isolated from Yellowstone National Park (Erdmann and Garrett, Mol Microbiol 85:1044-1056, 2012). Experimental studies of isolated genetic elements from this mixture revealed that SMV1 (S ulfolobus Monocauda Virus 1), a tailed spindle-shaped virus, can induce spacer acquisition in CRISPR loci of Sulfolobus species from a second coinfecting conjugative plasmid or virus (Erdmann and Garrett, Mol Microbiol 85:1044-1056, 2012; Erdmann et al. Mol Microbiol 91:900-917, 2014). Here we describe, firstly, the isolation of archaeal virus mixtures from terrestrial hot springs and the techniques used both to infect laboratory strains with these virus mixtures and to obtain purified virus particles. Secondly, we present the experimental conditions required for activating SMV1-induced spacer acquisition in two different Sulfolobus species.",
author = "Susanne Erdmann and Garrett, {Roger Antony}",
year = "2015",
doi = "10.1007/978-1-4939-2687-9_14",
language = "English",
isbn = "978-1-4939-2686-2",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "223--232",
editor = "Lundgren, {Magnus } and Charpentier, {Emmanuelle } and Fineran, {Peter C. }",
booktitle = "CRISPR",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - Archaeal viruses of the sulfolobales

T2 - isolation, infection, and CRISPR spacer acquisition

AU - Erdmann, Susanne

AU - Garrett, Roger Antony

PY - 2015

Y1 - 2015

N2 - Infection of archaea with phylogenetically diverse single viruses, performed in different laboratories, has failed to activate spacer acquisition into host CRISPR loci. The first successful uptake of archaeal de novo spacers was observed on infection of Sulfolobus solfataricus P2 with an environmental virus mixture isolated from Yellowstone National Park (Erdmann and Garrett, Mol Microbiol 85:1044-1056, 2012). Experimental studies of isolated genetic elements from this mixture revealed that SMV1 (S ulfolobus Monocauda Virus 1), a tailed spindle-shaped virus, can induce spacer acquisition in CRISPR loci of Sulfolobus species from a second coinfecting conjugative plasmid or virus (Erdmann and Garrett, Mol Microbiol 85:1044-1056, 2012; Erdmann et al. Mol Microbiol 91:900-917, 2014). Here we describe, firstly, the isolation of archaeal virus mixtures from terrestrial hot springs and the techniques used both to infect laboratory strains with these virus mixtures and to obtain purified virus particles. Secondly, we present the experimental conditions required for activating SMV1-induced spacer acquisition in two different Sulfolobus species.

AB - Infection of archaea with phylogenetically diverse single viruses, performed in different laboratories, has failed to activate spacer acquisition into host CRISPR loci. The first successful uptake of archaeal de novo spacers was observed on infection of Sulfolobus solfataricus P2 with an environmental virus mixture isolated from Yellowstone National Park (Erdmann and Garrett, Mol Microbiol 85:1044-1056, 2012). Experimental studies of isolated genetic elements from this mixture revealed that SMV1 (S ulfolobus Monocauda Virus 1), a tailed spindle-shaped virus, can induce spacer acquisition in CRISPR loci of Sulfolobus species from a second coinfecting conjugative plasmid or virus (Erdmann and Garrett, Mol Microbiol 85:1044-1056, 2012; Erdmann et al. Mol Microbiol 91:900-917, 2014). Here we describe, firstly, the isolation of archaeal virus mixtures from terrestrial hot springs and the techniques used both to infect laboratory strains with these virus mixtures and to obtain purified virus particles. Secondly, we present the experimental conditions required for activating SMV1-induced spacer acquisition in two different Sulfolobus species.

U2 - 10.1007/978-1-4939-2687-9_14

DO - 10.1007/978-1-4939-2687-9_14

M3 - Book chapter

C2 - 25981476

SN - 978-1-4939-2686-2

T3 - Methods in Molecular Biology

SP - 223

EP - 232

BT - CRISPR

A2 - Lundgren, Magnus

A2 - Charpentier, Emmanuelle

A2 - Fineran, Peter C.

PB - Springer

ER -

ID: 150197445