Cell cycle-regulated centers of DNA double-strand break repair
Research output: Contribution to journal › Journal article › Research › peer-review
In eukaryotes, homologous recombination is an important pathway for the repair of DNA double-strand breaks. We have studied this process in living cells in the yeast Saccharomyces cerevisiae using Rad52 as a cell biological marker. In response to DNA damage, Rad52 redistributes itself and forms foci specifically during S phase. We have shown previously that Rad52 foci are centers of DNA repair where multiple DNA double-strand breaks colocalize. Here we report a correlation between the timing of Rad52 focus formation and modification of the Rad52 protein. In addition, we show that the two ends of a double-strand break are held tightly together in the majority of cells. Interestingly, in a small but significant fraction of the S phase cells, the two ends of a break separate suggesting that mechanisms exist to reassociate and align these ends for proper DNA repair.
Original language | English |
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Journal | Cell Cycle |
Volume | 2 |
Issue number | 5 |
Pages (from-to) | 479-83 |
Number of pages | 5 |
ISSN | 1538-4101 |
Publication status | Published - 10 Sep 2003 |
- Cell Cycle, DNA Damage, DNA Repair, DNA-Binding Proteins, Rad52 DNA Repair and Recombination Protein, Recombination, Genetic, S Phase, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.
Research areas
ID: 184396723