Chitin and stress induced protein kinase activation
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
The assays described here are pertinent to protein kinase studies in any plant. They include an immunoblot phosphorylation/activation assay and an in-gel activity assay for MAP kinases (MPKs) using the general protein kinase substrate myelin basic protein. They also include a novel in-gel peptide substrate assay for Snf1-related kinase family 2 members (SnRK2s). This kinase family-specific assay overcomes some limitations of in-gel assays and permits the identification of different types of kinase activities in total protein extracts.
Original language | English |
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Title of host publication | Plant pattern recognition receptors : methods and protocols |
Editors | Libo Shan, Ping He |
Number of pages | 10 |
Publisher | Springer |
Publication date | 2017 |
Pages | 185-194 |
Chapter | 15 |
ISBN (Print) | 978-1-4939-6858-9 |
ISBN (Electronic) | 978-1-4939-6859-6 |
DOIs | |
Publication status | Published - 2017 |
Series | Methods in Molecular Biology |
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Volume | 1578 |
ISSN | 1064-3745 |
- Acryloylated peptide substrates, In-gel assays, Myelin basic protein
Research areas
ID: 181454851