Residual biomass from surfactin production is a source of arginase and adsorbed surfactin that is useful for environmental remediation
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Residual biomass from surfactin production is a source of arginase and adsorbed surfactin that is useful for environmental remediation. / Silveira, Thais de Carvalho; Gomes, Wyllerson Evaristo; Tonon, Giovana Chinaglia; Beatto, Thainá Godoy; Spogis, Nicolas; Cunha, Luiz Henrique Dallan; Lattaro, Bruno Pera; Nogueira, Alessandra Borin; Mendes, Renata Kelly; Alvarenga, Danillo Oliveira; Etchegaray, Augusto.
In: World Journal of Microbiology and Biotechnology, Vol. 37, No. 7, 123, 2021.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Residual biomass from surfactin production is a source of arginase and adsorbed surfactin that is useful for environmental remediation
AU - Silveira, Thais de Carvalho
AU - Gomes, Wyllerson Evaristo
AU - Tonon, Giovana Chinaglia
AU - Beatto, Thainá Godoy
AU - Spogis, Nicolas
AU - Cunha, Luiz Henrique Dallan
AU - Lattaro, Bruno Pera
AU - Nogueira, Alessandra Borin
AU - Mendes, Renata Kelly
AU - Alvarenga, Danillo Oliveira
AU - Etchegaray, Augusto
PY - 2021
Y1 - 2021
N2 - Lipopeptides are important secondary metabolites produced by microbes. They find applications in environmental decontamination and in the chemical, pharmaceutical and food industries. However, their production is expensive. In the present work we propose three strategies to lower the production costs of surfactin. First, the coproduction of surfactin and arginase in a single growth. Second, extract the fraction of surfactin that adsorbs to the biomass and is removed from the growth medium through centrifugation. Third, use microbial biomass for the remediation of organic and inorganic contaminants. The coproduction of surfactin and arginase was evaluated by factorial design experiments using the LB medium supplemented with arginine. The best conditions for surfactin production were 22 h of growth at 37 °C using LB supplemented with arginine 7.3 g/L. Almost similar conditions were found to produce highest levels of arginase, 24 h and 6.45 g/L arginine. Decontamination of phenol and copper from artificial samples was attained by treatment with residues from lipopeptide production. Thus, cell suspensions and wash-waters used to extract surfactin from the biomass. Cell suspensions were used to successfully remove hydroquinone. Cell suspensions and wash-waters containing surfactin were successfully used to recover copper from solution. Specific monitoring methods were used for phenol and metal solutions, respectively a biosensor based on tyrosinase and either atomic absorption flame ionization spectrometry or absorbance coupled to the Arduino™ platform. Therefore, we report three alternative strategies to lower the production costs in lipopeptide production, which include the effective recovery of copper and phenol from contaminated waters using residues from surfactin production.
AB - Lipopeptides are important secondary metabolites produced by microbes. They find applications in environmental decontamination and in the chemical, pharmaceutical and food industries. However, their production is expensive. In the present work we propose three strategies to lower the production costs of surfactin. First, the coproduction of surfactin and arginase in a single growth. Second, extract the fraction of surfactin that adsorbs to the biomass and is removed from the growth medium through centrifugation. Third, use microbial biomass for the remediation of organic and inorganic contaminants. The coproduction of surfactin and arginase was evaluated by factorial design experiments using the LB medium supplemented with arginine. The best conditions for surfactin production were 22 h of growth at 37 °C using LB supplemented with arginine 7.3 g/L. Almost similar conditions were found to produce highest levels of arginase, 24 h and 6.45 g/L arginine. Decontamination of phenol and copper from artificial samples was attained by treatment with residues from lipopeptide production. Thus, cell suspensions and wash-waters used to extract surfactin from the biomass. Cell suspensions were used to successfully remove hydroquinone. Cell suspensions and wash-waters containing surfactin were successfully used to recover copper from solution. Specific monitoring methods were used for phenol and metal solutions, respectively a biosensor based on tyrosinase and either atomic absorption flame ionization spectrometry or absorbance coupled to the Arduino™ platform. Therefore, we report three alternative strategies to lower the production costs in lipopeptide production, which include the effective recovery of copper and phenol from contaminated waters using residues from surfactin production.
U2 - 10.1007/s11274-021-03094-3
DO - 10.1007/s11274-021-03094-3
M3 - Journal article
C2 - 34160683
VL - 37
JO - World Journal of Microbiology and Biotechnology
JF - World Journal of Microbiology and Biotechnology
SN - 0959-3993
IS - 7
M1 - 123
ER -
ID: 272640105