The Hippo signalling pathway coordinates organ growth and limits developmental variability by controlling dilp8 expression

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The Hippo signalling pathway coordinates organ growth and limits developmental variability by controlling dilp8 expression. / Boone, Emilie; Colombani, Julien; Andersen, Ditte S; Léopold, Pierre.

In: Nature Communications, Vol. 7, 22.11.2016, p. 13505.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Boone, E, Colombani, J, Andersen, DS & Léopold, P 2016, 'The Hippo signalling pathway coordinates organ growth and limits developmental variability by controlling dilp8 expression', Nature Communications, vol. 7, pp. 13505. https://doi.org/10.1038/ncomms13505

APA

Boone, E., Colombani, J., Andersen, D. S., & Léopold, P. (2016). The Hippo signalling pathway coordinates organ growth and limits developmental variability by controlling dilp8 expression. Nature Communications, 7, 13505. https://doi.org/10.1038/ncomms13505

Vancouver

Boone E, Colombani J, Andersen DS, Léopold P. The Hippo signalling pathway coordinates organ growth and limits developmental variability by controlling dilp8 expression. Nature Communications. 2016 Nov 22;7:13505. https://doi.org/10.1038/ncomms13505

Author

Boone, Emilie ; Colombani, Julien ; Andersen, Ditte S ; Léopold, Pierre. / The Hippo signalling pathway coordinates organ growth and limits developmental variability by controlling dilp8 expression. In: Nature Communications. 2016 ; Vol. 7. pp. 13505.

Bibtex

@article{6448890edb304b278360c76822c94cd6,
title = "The Hippo signalling pathway coordinates organ growth and limits developmental variability by controlling dilp8 expression",
abstract = "Coordination of organ growth during development is required to generate fit individuals with fixed proportions. We recently identified Drosophila Dilp8 as a key hormone in coupling organ growth with animal maturation. In addition, dilp8 mutant flies exhibit elevated fluctuating asymmetry (FA) demonstrating a function for Dilp8 in ensuring developmental stability. The signals regulating Dilp8 activity during normal development are not yet known. Here, we show that the transcriptional co-activators of the Hippo (Hpo) pathway, Yorkie (Yki, YAP/TAZ) and its DNA-binding partner Scalloped (Sd), directly regulate dilp8 expression through a Hpo-responsive element (HRE) in the dilp8 promoter. We further demonstrate that mutation of the HRE by genome-editing results in animals with increased FA, thereby mimicking full dilp8 loss of function. Therefore, our results indicate that growth coordination of organs is connected to their growth status through a feedback loop involving Hpo and Dilp8 signalling pathways.",
keywords = "Animals, Cell Line, Drosophila Proteins/genetics, Drosophila melanogaster/genetics, Gene Deletion, Gene Editing, Gene Expression Regulation/physiology, Genotype, Intercellular Signaling Peptides and Proteins/genetics, Intracellular Signaling Peptides and Proteins/genetics, Larva/genetics, Protein-Serine-Threonine Kinases/genetics, Signal Transduction",
author = "Emilie Boone and Julien Colombani and Andersen, {Ditte S} and Pierre L{\'e}opold",
year = "2016",
month = nov,
day = "22",
doi = "10.1038/ncomms13505",
language = "English",
volume = "7",
pages = "13505",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - The Hippo signalling pathway coordinates organ growth and limits developmental variability by controlling dilp8 expression

AU - Boone, Emilie

AU - Colombani, Julien

AU - Andersen, Ditte S

AU - Léopold, Pierre

PY - 2016/11/22

Y1 - 2016/11/22

N2 - Coordination of organ growth during development is required to generate fit individuals with fixed proportions. We recently identified Drosophila Dilp8 as a key hormone in coupling organ growth with animal maturation. In addition, dilp8 mutant flies exhibit elevated fluctuating asymmetry (FA) demonstrating a function for Dilp8 in ensuring developmental stability. The signals regulating Dilp8 activity during normal development are not yet known. Here, we show that the transcriptional co-activators of the Hippo (Hpo) pathway, Yorkie (Yki, YAP/TAZ) and its DNA-binding partner Scalloped (Sd), directly regulate dilp8 expression through a Hpo-responsive element (HRE) in the dilp8 promoter. We further demonstrate that mutation of the HRE by genome-editing results in animals with increased FA, thereby mimicking full dilp8 loss of function. Therefore, our results indicate that growth coordination of organs is connected to their growth status through a feedback loop involving Hpo and Dilp8 signalling pathways.

AB - Coordination of organ growth during development is required to generate fit individuals with fixed proportions. We recently identified Drosophila Dilp8 as a key hormone in coupling organ growth with animal maturation. In addition, dilp8 mutant flies exhibit elevated fluctuating asymmetry (FA) demonstrating a function for Dilp8 in ensuring developmental stability. The signals regulating Dilp8 activity during normal development are not yet known. Here, we show that the transcriptional co-activators of the Hippo (Hpo) pathway, Yorkie (Yki, YAP/TAZ) and its DNA-binding partner Scalloped (Sd), directly regulate dilp8 expression through a Hpo-responsive element (HRE) in the dilp8 promoter. We further demonstrate that mutation of the HRE by genome-editing results in animals with increased FA, thereby mimicking full dilp8 loss of function. Therefore, our results indicate that growth coordination of organs is connected to their growth status through a feedback loop involving Hpo and Dilp8 signalling pathways.

KW - Animals

KW - Cell Line

KW - Drosophila Proteins/genetics

KW - Drosophila melanogaster/genetics

KW - Gene Deletion

KW - Gene Editing

KW - Gene Expression Regulation/physiology

KW - Genotype

KW - Intercellular Signaling Peptides and Proteins/genetics

KW - Intracellular Signaling Peptides and Proteins/genetics

KW - Larva/genetics

KW - Protein-Serine-Threonine Kinases/genetics

KW - Signal Transduction

U2 - 10.1038/ncomms13505

DO - 10.1038/ncomms13505

M3 - Journal article

C2 - 27874005

VL - 7

SP - 13505

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

ER -

ID: 212683216