Molecular cloning of a novel, putative G protein-coupled receptor from sea anemones structurally related to members of the FSH, TSH, LH/CG receptor family from mammals
Research output: Contribution to journal › Journal article › Research › peer-review
Using oligonucleotide probes derived from consensus sequences of known vertebrate and invertebrate G protein-coupled receptors, we have cloned the cDNA for a presumed G protein-coupled receptor from sea anemones. This receptor shows a striking structural homology with members of the glycoprotein hormone (FSH, TSH, LH/CG) receptor family from mammals, including a very large, extracellular N terminus (18-25% sequence identity) and a 7 transmembrane region (44-48% sequence identity). As with the mammalian glycoprotein hormone receptor genes, the sea anemone receptor gene yields transcripts which can be alternatively spliced, thereby yielding a shortened receptor variant only containing the large extracellular (soluble) N terminus. All this is strong evidence that the putative glycoprotein hormone receptor from sea anemones is evolutionarily related to those from mammals. This is the first report showing that a putative glycoprotein hormone receptor occurs in invertebrates.
Original language | English |
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Journal | Biochemical and Biophysical Research Communications |
Volume | 197 |
Issue number | 3 |
Pages (from-to) | 1062-9 |
Number of pages | 8 |
ISSN | 0006-291X |
DOIs | |
Publication status | Published - 30 Dec 1993 |
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA Primers, Exons, GTP-Binding Proteins, Gene Library, Mammals, Molecular Sequence Data, Oligonucleotide Probes, Polymerase Chain Reaction, Rats, Receptors, Cell Surface, Receptors, FSH, Receptors, LH, Receptors, Thyrotropin, Repetitive Sequences, Nucleic Acid, Sea Anemones, Sequence Homology, Amino Acid
Research areas
ID: 33513847