Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta
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Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta. / Kanost, Michael R; Arrese, Estela L; Cao, Xiaolong; Chen, Yun-Ru; Chellapilla, Sanjay; Goldsmith, Marian R; Grosse-Wilde, Ewald; Heckel, David G; Herndon, Nicolae; Jiang, Haobo; Papanicolaou, Alexie; Qu, Jiaxin; Soulages, Jose L; Vogel, Heiko; Walters, James; Waterhouse, Robert M; Ahn, Seung-Joon; Almeida, Francisca C; An, Chunju; Aqrawi, Peshtewani; Bretschneider, Anne; Bryant, William B; Bucks, Sascha; Chao, Hsu; Chevignon, Germain; Christen, Jayne M; Clarke, David F; Dittmer, Neal T; Ferguson, Laura C F; Garavelou, Spyridoula; Gordon, Karl H J; Gunaratna, Ramesh T; Han, Yi; Hauser, Frank; He, Yan; Heidel-Fischer, Hanna; Hirsh, Ariana; Hu, Yingxia; Jiang, Hongbo; Kalra, Divya; Klinner, Christian; König, Christopher; Kovar, Christie; Kroll, Ashley R; Kuwar, Suyog S; Lee, Sandy L; Lehman, Rüdiger; Li, Kai; Li, Zhaofei; Liang, Hanquan; Lovelace, Shanna; Lu, Zhiqiang; Mansfield, Jennifer H; McCulloch, Kyle J; Mathew, Tittu; Morton, Brian; Muzny, Donna M; Neunemann, David; Ongeri, Fiona; Pauchet, Yannick; Pu, Ling-Ling; Pyrousis, Ioannis; Rao, Xiang-Jun; Redding, Amanda; Roesel, Charles; Sanchez-Gracia, Alejandro; Schaack, Sarah; Shukla, Aditi; Tetreau, Guillaume; Wang, Yang; Xiong, Guang-Hua; Traut, Walther; Walsh, Tom K; Worley, Kim C; Wu, Di; Wu, Wenbi; Wu, Yuan-Qing; Zhang, Xiufeng; Zou, Zhen; Zucker, Hannah; Briscoe, Adriana D; Burmester, Thorsten; Clem, Rollie J; Feyereisen, René; Grimmelikhuijzen, Cornelis; Hamodrakas, Stavros J; Hansson, Bill S; Huguet, Elisabeth; Jermiin, Lars S; Lan, Que; Lehman, Herman K; Lorenzen, Marce; Merzendorfer, Hans; Michalopoulos, Ioannis; Morton, David B; Muthukrishnan, Subbaratnam; Oakeshott, John G; Palmer, Will; Park, Yoonseong; Passarelli, A Lorena; Rozas, Julio; Schwartz, Lawrence M; Smith, Wendy; Southgate, Agnes; Vilcinskas, Andreas; Vogt, Richard; Wang, Ping; Werren, John; Yu, Xiao-Qiang; Zhou, Jing-Jiang; Brown, Susan J; Scherer, Steven E; Richards, Stephen; Blissard, Gary W.
In: Insect Biochemistry and Molecular Biology, Vol. 76, 2016, p. 118-147.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta
AU - Kanost, Michael R
AU - Arrese, Estela L
AU - Cao, Xiaolong
AU - Chen, Yun-Ru
AU - Chellapilla, Sanjay
AU - Goldsmith, Marian R
AU - Grosse-Wilde, Ewald
AU - Heckel, David G
AU - Herndon, Nicolae
AU - Jiang, Haobo
AU - Papanicolaou, Alexie
AU - Qu, Jiaxin
AU - Soulages, Jose L
AU - Vogel, Heiko
AU - Walters, James
AU - Waterhouse, Robert M
AU - Ahn, Seung-Joon
AU - Almeida, Francisca C
AU - An, Chunju
AU - Aqrawi, Peshtewani
AU - Bretschneider, Anne
AU - Bryant, William B
AU - Bucks, Sascha
AU - Chao, Hsu
AU - Chevignon, Germain
AU - Christen, Jayne M
AU - Clarke, David F
AU - Dittmer, Neal T
AU - Ferguson, Laura C F
AU - Garavelou, Spyridoula
AU - Gordon, Karl H J
AU - Gunaratna, Ramesh T
AU - Han, Yi
AU - Hauser, Frank
AU - He, Yan
AU - Heidel-Fischer, Hanna
AU - Hirsh, Ariana
AU - Hu, Yingxia
AU - Jiang, Hongbo
AU - Kalra, Divya
AU - Klinner, Christian
AU - König, Christopher
AU - Kovar, Christie
AU - Kroll, Ashley R
AU - Kuwar, Suyog S
AU - Lee, Sandy L
AU - Lehman, Rüdiger
AU - Li, Kai
AU - Li, Zhaofei
AU - Liang, Hanquan
AU - Lovelace, Shanna
AU - Lu, Zhiqiang
AU - Mansfield, Jennifer H
AU - McCulloch, Kyle J
AU - Mathew, Tittu
AU - Morton, Brian
AU - Muzny, Donna M
AU - Neunemann, David
AU - Ongeri, Fiona
AU - Pauchet, Yannick
AU - Pu, Ling-Ling
AU - Pyrousis, Ioannis
AU - Rao, Xiang-Jun
AU - Redding, Amanda
AU - Roesel, Charles
AU - Sanchez-Gracia, Alejandro
AU - Schaack, Sarah
AU - Shukla, Aditi
AU - Tetreau, Guillaume
AU - Wang, Yang
AU - Xiong, Guang-Hua
AU - Traut, Walther
AU - Walsh, Tom K
AU - Worley, Kim C
AU - Wu, Di
AU - Wu, Wenbi
AU - Wu, Yuan-Qing
AU - Zhang, Xiufeng
AU - Zou, Zhen
AU - Zucker, Hannah
AU - Briscoe, Adriana D
AU - Burmester, Thorsten
AU - Clem, Rollie J
AU - Feyereisen, René
AU - Grimmelikhuijzen, Cornelis
AU - Hamodrakas, Stavros J
AU - Hansson, Bill S
AU - Huguet, Elisabeth
AU - Jermiin, Lars S
AU - Lan, Que
AU - Lehman, Herman K
AU - Lorenzen, Marce
AU - Merzendorfer, Hans
AU - Michalopoulos, Ioannis
AU - Morton, David B
AU - Muthukrishnan, Subbaratnam
AU - Oakeshott, John G
AU - Palmer, Will
AU - Park, Yoonseong
AU - Passarelli, A Lorena
AU - Rozas, Julio
AU - Schwartz, Lawrence M
AU - Smith, Wendy
AU - Southgate, Agnes
AU - Vilcinskas, Andreas
AU - Vogt, Richard
AU - Wang, Ping
AU - Werren, John
AU - Yu, Xiao-Qiang
AU - Zhou, Jing-Jiang
AU - Brown, Susan J
AU - Scherer, Steven E
AU - Richards, Stephen
AU - Blissard, Gary W
N1 - Copyright © 2016 Elsevier Ltd. All rights reserved.
PY - 2016
Y1 - 2016
N2 - Manduca sexta, known as the tobacco hornworm or Carolina sphinx moth, is a lepidopteran insect that is used extensively as a model system for research in insect biochemistry, physiology, neurobiology, development, and immunity. One important benefit of this species as an experimental model is its extremely large size, reaching more than 10 g in the larval stage. M. sexta larvae feed on solanaceous plants and thus must tolerate a substantial challenge from plant allelochemicals, including nicotine. We report the sequence and annotation of the M. sexta genome, and a survey of gene expression in various tissues and developmental stages. The Msex_1.0 genome assembly resulted in a total genome size of 419.4 Mbp. Repetitive sequences accounted for 25.8% of the assembled genome. The official gene set is comprised of 15,451 protein-coding genes, of which 2498 were manually curated. Extensive RNA-seq data from many tissues and developmental stages were used to improve gene models and for insights into gene expression patterns. Genome wide synteny analysis indicated a high level of macrosynteny in the Lepidoptera. Annotation and analyses were carried out for gene families involved in a wide spectrum of biological processes, including apoptosis, vacuole sorting, growth and development, structures of exoskeleton, egg shells, and muscle, vision, chemosensation, ion channels, signal transduction, neuropeptide signaling, neurotransmitter synthesis and transport, nicotine tolerance, lipid metabolism, and immunity. This genome sequence, annotation, and analysis provide an important new resource from a well-studied model insect species and will facilitate further biochemical and mechanistic experimental studies of many biological systems in insects.
AB - Manduca sexta, known as the tobacco hornworm or Carolina sphinx moth, is a lepidopteran insect that is used extensively as a model system for research in insect biochemistry, physiology, neurobiology, development, and immunity. One important benefit of this species as an experimental model is its extremely large size, reaching more than 10 g in the larval stage. M. sexta larvae feed on solanaceous plants and thus must tolerate a substantial challenge from plant allelochemicals, including nicotine. We report the sequence and annotation of the M. sexta genome, and a survey of gene expression in various tissues and developmental stages. The Msex_1.0 genome assembly resulted in a total genome size of 419.4 Mbp. Repetitive sequences accounted for 25.8% of the assembled genome. The official gene set is comprised of 15,451 protein-coding genes, of which 2498 were manually curated. Extensive RNA-seq data from many tissues and developmental stages were used to improve gene models and for insights into gene expression patterns. Genome wide synteny analysis indicated a high level of macrosynteny in the Lepidoptera. Annotation and analyses were carried out for gene families involved in a wide spectrum of biological processes, including apoptosis, vacuole sorting, growth and development, structures of exoskeleton, egg shells, and muscle, vision, chemosensation, ion channels, signal transduction, neuropeptide signaling, neurotransmitter synthesis and transport, nicotine tolerance, lipid metabolism, and immunity. This genome sequence, annotation, and analysis provide an important new resource from a well-studied model insect species and will facilitate further biochemical and mechanistic experimental studies of many biological systems in insects.
KW - Journal Article
U2 - 10.1016/j.ibmb.2016.07.005
DO - 10.1016/j.ibmb.2016.07.005
M3 - Journal article
C2 - 27522922
VL - 76
SP - 118
EP - 147
JO - Insect Biochemistry and Molecular Biology
JF - Insect Biochemistry and Molecular Biology
SN - 0965-1748
ER -
ID: 165395342