Revised genomic consensus for the hypermethylated CpG island region of the human L1 transposon and integration sites of full length L1 elements from recombinant clones made using methylation-tolerant host strains.
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Revised genomic consensus for the hypermethylated CpG island region of the human L1 transposon and integration sites of full length L1 elements from recombinant clones made using methylation-tolerant host strains. / Crowther, P J; Doherty, J P; Linsenmeyer, M E; Williamson, M R; Woodcock, D M.
In: Nucleic Acids Research, Vol. 19, No. 9, 1991, p. 2395-401.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Revised genomic consensus for the hypermethylated CpG island region of the human L1 transposon and integration sites of full length L1 elements from recombinant clones made using methylation-tolerant host strains.
AU - Crowther, P J
AU - Doherty, J P
AU - Linsenmeyer, M E
AU - Williamson, M R
AU - Woodcock, D M
N1 - Keywords: Base Sequence; Cloning, Molecular; Consensus Sequence; DNA; DNA Transposable Elements; Dinucleoside Phosphates; Humans; Methylation; Molecular Sequence Data; Nucleic Acid Conformation; Promoter Regions (Genetics); RNA; RNA Polymerase III
PY - 1991
Y1 - 1991
N2 - Efficient recovery of clones from the 5' end of the human L1 dispersed repetitive elements necessitates the use of deletion mcr- host strains since this region contains a CpG island which is hypermethylated in vivo. Clones recovered with conventional mcr+ hosts seem to have been derived preferentially from L1 members which have accumulated mutations that have removed sites of methylation. We present a revised consensus from the 5' presumptive control region of these elements. This revised consensus contains a consensus RNA polymerase III promoter which would permit the synthesis of transcripts from the 5' end of full length L1 elements. Such potential transcripts are likely to exhibit a high degree of secondary structure. In addition, we have determined the flanking sequences for 6 full length L1 elements. The majority of full length L1 clones show no convincing evidence for target site duplication in the insertion site as commonly observed with truncated L1 elements. These data would be consistent with two mechanisms of integration of transposing L1 elements with different mechanisms predominating for full length and truncated elements. Udgivelsesdato: 1991-May-11
AB - Efficient recovery of clones from the 5' end of the human L1 dispersed repetitive elements necessitates the use of deletion mcr- host strains since this region contains a CpG island which is hypermethylated in vivo. Clones recovered with conventional mcr+ hosts seem to have been derived preferentially from L1 members which have accumulated mutations that have removed sites of methylation. We present a revised consensus from the 5' presumptive control region of these elements. This revised consensus contains a consensus RNA polymerase III promoter which would permit the synthesis of transcripts from the 5' end of full length L1 elements. Such potential transcripts are likely to exhibit a high degree of secondary structure. In addition, we have determined the flanking sequences for 6 full length L1 elements. The majority of full length L1 clones show no convincing evidence for target site duplication in the insertion site as commonly observed with truncated L1 elements. These data would be consistent with two mechanisms of integration of transposing L1 elements with different mechanisms predominating for full length and truncated elements. Udgivelsesdato: 1991-May-11
M3 - Journal article
C2 - 1710354
VL - 19
SP - 2395
EP - 2401
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 9
ER -
ID: 3046348