The Hippo signalling pathway coordinates organ growth and limits developmental variability by controlling dilp8 expression
Research output: Contribution to journal › Journal article › Research › peer-review
Coordination of organ growth during development is required to generate fit individuals with fixed proportions. We recently identified Drosophila Dilp8 as a key hormone in coupling organ growth with animal maturation. In addition, dilp8 mutant flies exhibit elevated fluctuating asymmetry (FA) demonstrating a function for Dilp8 in ensuring developmental stability. The signals regulating Dilp8 activity during normal development are not yet known. Here, we show that the transcriptional co-activators of the Hippo (Hpo) pathway, Yorkie (Yki, YAP/TAZ) and its DNA-binding partner Scalloped (Sd), directly regulate dilp8 expression through a Hpo-responsive element (HRE) in the dilp8 promoter. We further demonstrate that mutation of the HRE by genome-editing results in animals with increased FA, thereby mimicking full dilp8 loss of function. Therefore, our results indicate that growth coordination of organs is connected to their growth status through a feedback loop involving Hpo and Dilp8 signalling pathways.
Original language | English |
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Journal | Nature Communications |
Volume | 7 |
Pages (from-to) | 13505 |
ISSN | 2041-1723 |
DOIs | |
Publication status | Published - 22 Nov 2016 |
Externally published | Yes |
- Animals, Cell Line, Drosophila Proteins/genetics, Drosophila melanogaster/genetics, Gene Deletion, Gene Editing, Gene Expression Regulation/physiology, Genotype, Intercellular Signaling Peptides and Proteins/genetics, Intracellular Signaling Peptides and Proteins/genetics, Larva/genetics, Protein-Serine-Threonine Kinases/genetics, Signal Transduction
Research areas
ID: 212683216