Ecdysone-dependent feedback regulation of prothoracicotropic hormone controls the timing of developmental maturation
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Ecdysone-dependent feedback regulation of prothoracicotropic hormone controls the timing of developmental maturation. / Christensen, Christian F.; Koyama, Takashi; Nagy, Stanislav; Danielsen, E. Thomas; Texada, Michael J.; Halberg, Kenneth A.; Rewitz, Kim.
In: Development, Vol. 147, No. 14, dev188110, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Ecdysone-dependent feedback regulation of prothoracicotropic hormone controls the timing of developmental maturation
AU - Christensen, Christian F.
AU - Koyama, Takashi
AU - Nagy, Stanislav
AU - Danielsen, E. Thomas
AU - Texada, Michael J.
AU - Halberg, Kenneth A.
AU - Rewitz, Kim
PY - 2020
Y1 - 2020
N2 - The activation of a neuroendocrine system that induces a surge in steroid production is a conserved initiator of the juvenile-to-adult transition in many animals. The trigger for maturation is the secretion of brain-derived neuropeptides, yet the mechanisms controlling the timely onset of this event remain ill-defined. Here, we show that a regulatory feedback circuit controlling the Drosophila neuropeptide Prothoracicotropic hormone (PTTH) triggers maturation onset. We identify the Ecdysone Receptor (EcR) in the PTTH-expressing neurons (PTTHn) as a regulator of developmental maturation onset. Loss of EcR in these PTTHn impairs PTTH signaling, which delays maturation. We find that the steroid ecdysone dose-dependently affects Ptth transcription, promoting its expression at lower concentrations and inhibiting it at higher concentrations. Our findings indicate the existence of a feedback circuit in which rising ecdysone levels trigger, via EcR activity in the PTTHn, the PTTH surge that generates the maturation-inducing ecdysone peak toward the end of larval development. Because steroid feedback is also known to control the vertebrate maturation-inducing hypothalamicpituitary-gonadal axis, our findings suggest an overall conservation of the feedback-regulatory neuroendocrine circuitry that controls the timing of maturation initiation.
AB - The activation of a neuroendocrine system that induces a surge in steroid production is a conserved initiator of the juvenile-to-adult transition in many animals. The trigger for maturation is the secretion of brain-derived neuropeptides, yet the mechanisms controlling the timely onset of this event remain ill-defined. Here, we show that a regulatory feedback circuit controlling the Drosophila neuropeptide Prothoracicotropic hormone (PTTH) triggers maturation onset. We identify the Ecdysone Receptor (EcR) in the PTTH-expressing neurons (PTTHn) as a regulator of developmental maturation onset. Loss of EcR in these PTTHn impairs PTTH signaling, which delays maturation. We find that the steroid ecdysone dose-dependently affects Ptth transcription, promoting its expression at lower concentrations and inhibiting it at higher concentrations. Our findings indicate the existence of a feedback circuit in which rising ecdysone levels trigger, via EcR activity in the PTTHn, the PTTH surge that generates the maturation-inducing ecdysone peak toward the end of larval development. Because steroid feedback is also known to control the vertebrate maturation-inducing hypothalamicpituitary-gonadal axis, our findings suggest an overall conservation of the feedback-regulatory neuroendocrine circuitry that controls the timing of maturation initiation.
KW - Drosophila
KW - Ecdysone
KW - Maturation
KW - Prothoracicotropic
KW - Ptth
KW - Steroid
U2 - 10.1242/dev.188110
DO - 10.1242/dev.188110
M3 - Journal article
C2 - 32631830
AN - SCOPUS:85088682108
VL - 147
JO - Development
JF - Development
SN - 0950-1991
IS - 14
M1 - dev188110
ER -
ID: 247984587