A Novel Fluorescence-Based Screen for Inhibitors of the Initiation of DNA Replication in Bacteria
Research output: Contribution to journal › Journal article › Research › peer-review
BACKGROUND: One of many strategies to overcome antibiotic resistance is the discovery of compounds targeting cellular processes, which have not yet been exploited.
METHODS AND MATERIALS: Using various genetic tools, we constructed a novel high throughput, cell based, fluorescence screen for inhibitors of chromosome replication initiation in bacteria.
RESULTS: The screen was validated by expression of an intra-cellular cyclic peptide interfering with the initiator protein DnaA and by over-expression of the negative initiation regulator SeqA. We also demonstrated that neither tetracycline nor ciprofloxacin triggers a false positive result. Finally, 400 extracts isolated mainly from filamentous actinomycetes were subjected to the screen.
CONCLUSION: We concluded that the presented screen is applicable for identifying putative inhibitors of DNA replication initiation in a high throughput setup.
Original language | English |
---|---|
Journal | Current Drug Discovery Technologies |
Volume | 16 |
Issue number | 3 |
Pages (from-to) | 272-277 |
ISSN | 1570-1638 |
DOIs | |
Publication status | Published - 2019 |
ID: 196438494