CRISPR-Cas systems are widespread accessory elements across bacterial and archaeal plasmids
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CRISPR-Cas systems are widespread accessory elements across bacterial and archaeal plasmids. / Pinilla-Redondo, Rafael; Russel, Jakob; Mayo-Muñoz, David; Shah, Shiraz A.; Garrett, Roger A.; Nesme, Joseph; Madsen, Jonas S.; Fineran, Peter C.; Sørensen, Søren J.
In: Nucleic Acids Research, Vol. 50, No. 8, 2022, p. 4315-4328.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - CRISPR-Cas systems are widespread accessory elements across bacterial and archaeal plasmids
AU - Pinilla-Redondo, Rafael
AU - Russel, Jakob
AU - Mayo-Muñoz, David
AU - Shah, Shiraz A.
AU - Garrett, Roger A.
AU - Nesme, Joseph
AU - Madsen, Jonas S.
AU - Fineran, Peter C.
AU - Sørensen, Søren J.
N1 - © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.
PY - 2022
Y1 - 2022
N2 - Many prokaryotes encode CRISPR-Cas systems as immune protection against mobile genetic elements (MGEs), yet a number of MGEs also harbor CRISPR-Cas components. With a few exceptions, CRISPR-Cas loci encoded on MGEs are uncharted and a comprehensive analysis of their distribution, prevalence, diversity, and function is lacking. Here, we systematically investigated CRISPR-Cas loci across the largest curated collection of natural bacterial and archaeal plasmids. CRISPR-Cas loci are widely but heterogeneously distributed across plasmids and, in comparison to host chromosomes, their mean prevalence per Mbp is higher and their distribution is distinct. Furthermore, the spacer content of plasmid CRISPRs exhibits a strong targeting bias towards other plasmids, while chromosomal arrays are enriched with virus-targeting spacers. These contrasting targeting preferences highlight the genetic independence of plasmids and suggest a major role for mediating plasmid-plasmid conflicts. Altogether, CRISPR-Cas are frequent accessory components of many plasmids, which is an overlooked phenomenon that possibly facilitates their dissemination across microbiomes.
AB - Many prokaryotes encode CRISPR-Cas systems as immune protection against mobile genetic elements (MGEs), yet a number of MGEs also harbor CRISPR-Cas components. With a few exceptions, CRISPR-Cas loci encoded on MGEs are uncharted and a comprehensive analysis of their distribution, prevalence, diversity, and function is lacking. Here, we systematically investigated CRISPR-Cas loci across the largest curated collection of natural bacterial and archaeal plasmids. CRISPR-Cas loci are widely but heterogeneously distributed across plasmids and, in comparison to host chromosomes, their mean prevalence per Mbp is higher and their distribution is distinct. Furthermore, the spacer content of plasmid CRISPRs exhibits a strong targeting bias towards other plasmids, while chromosomal arrays are enriched with virus-targeting spacers. These contrasting targeting preferences highlight the genetic independence of plasmids and suggest a major role for mediating plasmid-plasmid conflicts. Altogether, CRISPR-Cas are frequent accessory components of many plasmids, which is an overlooked phenomenon that possibly facilitates their dissemination across microbiomes.
U2 - 10.1093/nar/gkab859
DO - 10.1093/nar/gkab859
M3 - Journal article
C2 - 34606604
VL - 50
SP - 4315
EP - 4328
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 8
ER -
ID: 281224711