Development of a novel SRAP-SCAR marker for rapid identification of lager and ale types in brewer's yeast

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Development of a novel SRAP-SCAR marker for rapid identification of lager and ale types in brewer's yeast. / Liu, Jun; Cai, Guolin; Li, Xiaomin; Lu, Jian.

In: Molecular Biology Reports, Vol. 50, 2023.

Research output: Contribution to journal › Letter › Research › peer-review

Harvard

Liu, J, Cai, G, Li, X & Lu, J 2023, 'Development of a novel SRAP-SCAR marker for rapid identification of lager and ale types in brewer's yeast', Molecular Biology Reports, vol. 50. https://doi.org/10.1007/s11033-022-08058-w

APA

Liu, J., Cai, G., Li, X., & Lu, J. (2023). Development of a novel SRAP-SCAR marker for rapid identification of lager and ale types in brewer's yeast. Molecular Biology Reports, 50. https://doi.org/10.1007/s11033-022-08058-w

Vancouver

Liu J, Cai G, Li X, Lu J. Development of a novel SRAP-SCAR marker for rapid identification of lager and ale types in brewer's yeast. Molecular Biology Reports. 2023;50. https://doi.org/10.1007/s11033-022-08058-w

Author

Liu, Jun ; Cai, Guolin ; Li, Xiaomin ; Lu, Jian. / Development of a novel SRAP-SCAR marker for rapid identification of lager and ale types in brewer's yeast. In: Molecular Biology Reports. 2023 ; Vol. 50.

Bibtex

@article{4c2f1ed7734740b19a09b234addae866,
title = "Development of a novel SRAP-SCAR marker for rapid identification of lager and ale types in brewer's yeast",
abstract = "Background Beer is a globally consumed and universally popular beverage. According to the fermentation conditions of brewer's yeast, ale yeast and lager yeast are the two major varieties. Normal phenotypic and genotypic approaches are insufficient and time-consuming for identifying these two forms of yeast. Therefore, a method for the rapid and cost-effective identification of lager and ale-type brewer's yeasts is necessary. Methods and results In this study, we analysed the genetic diversity of 23 industrial brewer's yeasts from around the world using sequence-related amplified polymorphism (SRAP) markers and produced stable sequence characteristic amplification region (SCAR) markers. The specific DNA fragments identified by the SRAP marker were sequenced and primers were constructed; the resultant SCAR marker (757 bp) was then confirmed against the indicated brewer's yeast type. Conclusion The development of SRAP-SCAR marker is more economical, simple, and fast compared to morphological markers.",
keywords = "Beer, Lager yeast, Ale yeast, SRAP, SCAR, DIVERSITY, DNA",
author = "Jun Liu and Guolin Cai and Xiaomin Li and Jian Lu",
year = "2023",
doi = "10.1007/s11033-022-08058-w",
language = "English",
volume = "50",
journal = "Molecular Biology Reports",
issn = "0301-4851",
publisher = "Springer",

}

RIS

TY - JOUR

T1 - Development of a novel SRAP-SCAR marker for rapid identification of lager and ale types in brewer's yeast

AU - Liu, Jun

AU - Cai, Guolin

AU - Li, Xiaomin

AU - Lu, Jian

PY - 2023

Y1 - 2023

N2 - Background Beer is a globally consumed and universally popular beverage. According to the fermentation conditions of brewer's yeast, ale yeast and lager yeast are the two major varieties. Normal phenotypic and genotypic approaches are insufficient and time-consuming for identifying these two forms of yeast. Therefore, a method for the rapid and cost-effective identification of lager and ale-type brewer's yeasts is necessary. Methods and results In this study, we analysed the genetic diversity of 23 industrial brewer's yeasts from around the world using sequence-related amplified polymorphism (SRAP) markers and produced stable sequence characteristic amplification region (SCAR) markers. The specific DNA fragments identified by the SRAP marker were sequenced and primers were constructed; the resultant SCAR marker (757 bp) was then confirmed against the indicated brewer's yeast type. Conclusion The development of SRAP-SCAR marker is more economical, simple, and fast compared to morphological markers.

AB - Background Beer is a globally consumed and universally popular beverage. According to the fermentation conditions of brewer's yeast, ale yeast and lager yeast are the two major varieties. Normal phenotypic and genotypic approaches are insufficient and time-consuming for identifying these two forms of yeast. Therefore, a method for the rapid and cost-effective identification of lager and ale-type brewer's yeasts is necessary. Methods and results In this study, we analysed the genetic diversity of 23 industrial brewer's yeasts from around the world using sequence-related amplified polymorphism (SRAP) markers and produced stable sequence characteristic amplification region (SCAR) markers. The specific DNA fragments identified by the SRAP marker were sequenced and primers were constructed; the resultant SCAR marker (757 bp) was then confirmed against the indicated brewer's yeast type. Conclusion The development of SRAP-SCAR marker is more economical, simple, and fast compared to morphological markers.

KW - Beer

KW - Lager yeast

KW - Ale yeast

KW - SRAP

KW - SCAR

KW - DIVERSITY

KW - DNA

U2 - 10.1007/s11033-022-08058-w

DO - 10.1007/s11033-022-08058-w

M3 - Letter

C2 - 36380024

VL - 50

JO - Molecular Biology Reports

JF - Molecular Biology Reports

SN - 0301-4851

ER -

ID: 329611160