Replication Protein Rep Provides Selective Advantage to Viruses in the Presence of CRISPR-Cas Immunity
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Replication Protein Rep Provides Selective Advantage to Viruses in the Presence of CRISPR-Cas Immunity. / Zhang, Weijia; Bhoobalan-Chitty, Yuvaraj; Zhai, Xichuan; Hui, Yan; Hansen, Lars Hestbjerg; Deng, Ling; Peng, Xu.
In: CRISPR Journal, Vol. 6, No. 1, 2023, p. 32-42.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Replication Protein Rep Provides Selective Advantage to Viruses in the Presence of CRISPR-Cas Immunity
AU - Zhang, Weijia
AU - Bhoobalan-Chitty, Yuvaraj
AU - Zhai, Xichuan
AU - Hui, Yan
AU - Hansen, Lars Hestbjerg
AU - Deng, Ling
AU - Peng, Xu
PY - 2023
Y1 - 2023
N2 - Anti-Clustered regularly interspaced small palindromic repeat (CRISPR) (Acr) phages cooperate to establish a successful infection in CRISPR-containing host. We report here the selective advantage provided by a replication initiator, Rep, toward cooperative host immunosuppression by viruses encoding Acrs. A rep knockout mutant (Δgp16) of Sulfolobus islandicus rod-shaped virus 2 produced around fourfold less virus in a CRISPR-null host, suggesting that Rep is the major replication initiator. In addition to Rep-dependent replication initiation from the viral genomic termini, we detected Rep-independent replication initiation from nonterminal sites. Intriguingly, despite the presence of Acrs, lack of Rep showed a profound effect on virus propagation in a host carrying CRISPR-Cas immunity. Accordingly, the co-infecting parental virus (rep-containing) outcompeted the Δgp16 mutant much more quickly in the CRISPR-containing host than in CRISPR-null host. Despite the nonessentiality, rep is carried by all known members of Rudiviridae, which is likely an evolutionary outcome driven by the ubiquitous presence of CRISPR-Cas in Sulfolobales.
AB - Anti-Clustered regularly interspaced small palindromic repeat (CRISPR) (Acr) phages cooperate to establish a successful infection in CRISPR-containing host. We report here the selective advantage provided by a replication initiator, Rep, toward cooperative host immunosuppression by viruses encoding Acrs. A rep knockout mutant (Δgp16) of Sulfolobus islandicus rod-shaped virus 2 produced around fourfold less virus in a CRISPR-null host, suggesting that Rep is the major replication initiator. In addition to Rep-dependent replication initiation from the viral genomic termini, we detected Rep-independent replication initiation from nonterminal sites. Intriguingly, despite the presence of Acrs, lack of Rep showed a profound effect on virus propagation in a host carrying CRISPR-Cas immunity. Accordingly, the co-infecting parental virus (rep-containing) outcompeted the Δgp16 mutant much more quickly in the CRISPR-containing host than in CRISPR-null host. Despite the nonessentiality, rep is carried by all known members of Rudiviridae, which is likely an evolutionary outcome driven by the ubiquitous presence of CRISPR-Cas in Sulfolobales.
U2 - 10.1089/crispr.2022.0037
DO - 10.1089/crispr.2022.0037
M3 - Journal article
C2 - 36576859
VL - 6
SP - 32
EP - 42
JO - CRISPR Journal
JF - CRISPR Journal
SN - 2573-1599
IS - 1
ER -
ID: 333308266