The Arabidopsis lue1 mutant defines a katanin p60 ortholog involved in hormonal control of microtubule orientation during cell growth.
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The lue1 mutant was previously isolated in a bio-imaging screen for Arabidopsis mutants exhibiting inappropriate regulation of an AtGA20ox1 promoter-luciferase reporter fusion. Here we show that lue1 is allelic to fra2, bot1 and erh3, and encodes a truncated katanin-like microtubule-severing protein (AtKSS). Complementation of lue1 with the wild-type AtKSS gene restored both wild-type stature and luciferase reporter levels. Hormonal responses of lue1 to ethylene and gibberellins revealed inappropriate cortical microtubule reorientation during cell growth. Moreover, a fusion between the AtKSS protein and GFP decorated cortical microtubules. A yeast two-hybrid screen with AtKSS as the bait identified proteins related to those involved in microtubule processing, including a katanin p80 subunit and a kinesin ortholog. These results indicate that AtKSS is involved in microtubule dynamics in response to plant hormones.
Original language | English |
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Journal | Journal of Cell Science |
Volume | 116 |
Issue number | Pt 5 |
Pages (from-to) | 791-801 |
Number of pages | 10 |
ISSN | 0021-9533 |
Publication status | Published - 2003 |
Bibliographical note
Keywords: Adenosine Triphosphatases; Amino Acid Sequence; Arabidopsis; Arabidopsis Proteins; Cell Division; Ethylenes; Gene Expression Regulation, Plant; Genes, Reporter; Gibberellins; Glucuronidase; Microfibrils; Microscopy, Polarization; Microtubules; Molecular Sequence Data; Mutation; Phenotype; Plant Growth Regulators; Plant Roots; RNA, Messenger; Sequence Homology, Amino Acid
ID: 6474679