The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor. / Christensen, P U; Davey, William John; Nielsen, O; Nielsen, Olaf.

In: Molecular and General Genetics MGG, Vol. 255, No. 2, 01.06.1997, p. 226-36.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Christensen, PU, Davey, WJ, Nielsen, O & Nielsen, O 1997, 'The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor', Molecular and General Genetics MGG, vol. 255, no. 2, pp. 226-36.

APA

Christensen, P. U., Davey, W. J., Nielsen, O., & Nielsen, O. (1997). The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor. Molecular and General Genetics MGG, 255(2), 226-36.

Vancouver

Christensen PU, Davey WJ, Nielsen O, Nielsen O. The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor. Molecular and General Genetics MGG. 1997 Jun 1;255(2):226-36.

Author

Christensen, P U ; Davey, William John ; Nielsen, O ; Nielsen, Olaf. / The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor. In: Molecular and General Genetics MGG. 1997 ; Vol. 255, No. 2. pp. 226-36.

Bibtex

@article{663e2efc410e4fc69dc189d3dd0e24eb,
title = "The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor",
abstract = "In the fission yeast Schizosaccharomyces pombe, cells of opposite mating type communicate via diffusible peptide pheromones prior to mating. We have cloned the S. pombe mam1 gene, which encodes a 1336-amino acid protein belonging to the ATP-binding cassette (ABC) superfamily. The mam1 gene is only expressed in M cells and the gene product is responsible for the secretion of the mating pheromone. M-factor, a nonapeptide that is S-farnesylated and carboxy-methylated on its C-terminal cysteine residue. The predicted Mam1 protein is highly homologous to mammalian multiple drug-resistance proteins and to the Saccharomyces cerevisiae STE6 gene product, which mediates export of a-factor mating pheromone. We show that STE6 can also mediate secretion of M-factor in S. pombe.",
keywords = "ATP-Binding Cassette Transporters, Amino Acid Sequence, Base Sequence, Cloning, Molecular, Fungal Proteins, Gene Expression Regulation, Fungal, Genetic Complementation Test, Glycoproteins, Guanine Nucleotide Exchange Factors, Molecular Sequence Data, Mutation, Nitrogen, P-Glycoprotein, Peptides, Pheromones, Saccharomyces cerevisiae Proteins, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Transcription, Genetic",
author = "Christensen, {P U} and Davey, {William John} and O Nielsen and Olaf Nielsen",
year = "1997",
month = jun,
day = "1",
language = "English",
volume = "255",
pages = "226--36",
journal = "Molecular General Genetics",
issn = "0026-8925",
publisher = "Springer Verlag",
number = "2",

}

RIS

TY - JOUR

T1 - The Schizosaccharomyces pombe mam1 gene encodes an ABC transporter mediating secretion of M-factor

AU - Christensen, P U

AU - Davey, William John

AU - Nielsen, O

AU - Nielsen, Olaf

PY - 1997/6/1

Y1 - 1997/6/1

N2 - In the fission yeast Schizosaccharomyces pombe, cells of opposite mating type communicate via diffusible peptide pheromones prior to mating. We have cloned the S. pombe mam1 gene, which encodes a 1336-amino acid protein belonging to the ATP-binding cassette (ABC) superfamily. The mam1 gene is only expressed in M cells and the gene product is responsible for the secretion of the mating pheromone. M-factor, a nonapeptide that is S-farnesylated and carboxy-methylated on its C-terminal cysteine residue. The predicted Mam1 protein is highly homologous to mammalian multiple drug-resistance proteins and to the Saccharomyces cerevisiae STE6 gene product, which mediates export of a-factor mating pheromone. We show that STE6 can also mediate secretion of M-factor in S. pombe.

AB - In the fission yeast Schizosaccharomyces pombe, cells of opposite mating type communicate via diffusible peptide pheromones prior to mating. We have cloned the S. pombe mam1 gene, which encodes a 1336-amino acid protein belonging to the ATP-binding cassette (ABC) superfamily. The mam1 gene is only expressed in M cells and the gene product is responsible for the secretion of the mating pheromone. M-factor, a nonapeptide that is S-farnesylated and carboxy-methylated on its C-terminal cysteine residue. The predicted Mam1 protein is highly homologous to mammalian multiple drug-resistance proteins and to the Saccharomyces cerevisiae STE6 gene product, which mediates export of a-factor mating pheromone. We show that STE6 can also mediate secretion of M-factor in S. pombe.

KW - ATP-Binding Cassette Transporters

KW - Amino Acid Sequence

KW - Base Sequence

KW - Cloning, Molecular

KW - Fungal Proteins

KW - Gene Expression Regulation, Fungal

KW - Genetic Complementation Test

KW - Glycoproteins

KW - Guanine Nucleotide Exchange Factors

KW - Molecular Sequence Data

KW - Mutation

KW - Nitrogen

KW - P-Glycoprotein

KW - Peptides

KW - Pheromones

KW - Saccharomyces cerevisiae Proteins

KW - Schizosaccharomyces

KW - Schizosaccharomyces pombe Proteins

KW - Sequence Analysis, DNA

KW - Sequence Homology, Amino Acid

KW - Transcription, Genetic

M3 - Journal article

C2 - 9236781

VL - 255

SP - 226

EP - 236

JO - Molecular General Genetics

JF - Molecular General Genetics

SN - 0026-8925

IS - 2

ER -

ID: 33576971