The Schizosaccharomyces pombe map1 gene encodes an SRF/MCM1-related protein required for P-cell specific gene expression
Research output: Contribution to journal › Journal article › Research › peer-review
Cells of Schizosaccharomyces pombe undergo mating and meiosis when starved for a nitrogen source. In this process a P and and M cell first mate to generate a diploid zygote, which subsequently enters meiosis and sporulates. The P mating type is controlled by the mat1-Pc gene at the mating type locus, together with a gene called map1. We show that these two functions are required for expression of the P-specific gene map3. We have cloned the map1 gene and show that it encodes a putative MADS-box containing transcription factor related to mammalian Serum Response Factor (SRF) and Saccharomyces cerevisiae MCM1. The Mat1-Pc protein contains a motif characteristic for proteins that interact with MADS-box factors, suggesting that Mat-Pc and Map1 may form a heterodimer that activates the P-specific map3 gene.
Original language | English |
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Journal | Molecular and General Genetics MGG |
Volume | 253 |
Issue number | 3 |
Pages (from-to) | 387-92 |
Number of pages | 6 |
ISSN | 0026-8925 |
Publication status | Published - 13 Dec 1996 |
- Amino Acid Sequence, Cloning, Molecular, DNA-Binding Proteins, Gene Expression Regulation, Fungal, Genes, Fungal, Genes, Mating Type, Fungal, High Mobility Group Proteins, Homeodomain Proteins, MCM1 Protein, Molecular Sequence Data, Nuclear Proteins, Restriction Mapping, Schizosaccharomyces, Schizosaccharomyces pombe Proteins, Sequence Homology, Amino Acid, Serum Response Factor, Transcription Factors
Research areas
ID: 33577028